The Mechanism of Tiaogan Formula in Preventing and Treating Coronary Heart Disease with Qi Stagnation and Blood Stasis Syndrome Based on"Disease-Syndrome-Formula"
CHEN Mingtai
CHEN Bohao
XU Chong
LIN Xiaojuan
CHENG Biwei
YI Yumeng
LIU Qiang
LI Rong
LUAN Jienan
Abstract：Objective:To explore the mechanism of the Tiaogan Formula in preventing and treating coronary heart disease with Qi stagnation and blood stasis syndrome.Methods:Based on the"disease-syndrome-formula"core concept,network analysis,bioinformatics and molecular docking verification,the effective material basis were systematically screened through the databases of traditional Chinese medicine syndromes,traditional Chinese medicine and diseases,and the drug targets were identified.Results:Tiaogan Formula mainly consisted of Xuefu Zhuyu Decoction,Chaihu Shugan Powder and Xiao Yao Powder.A total of 357 targets for Tiaogan Formula were identified through the TCMIP v2.0 platform.By the DisGeNET,GeneCards,OMIM,PharmGKB,TTD disease databases,and SymMap v2 syndrome database,424 targets for coronary heart disease with Qi stagnation and blood stasis syndrome were obtained.A target network of Tiaogan Formula in preventing and treating coronary heart disease with Qi stagnation and blood stasis syndrome was constructed,including 9 core targets,namely tumor necrosis factor(TNF),DNA methyltransferase 1(DNMT1),phosphatidylinositol 3-kinase catalytic subunit alpha(PIK3CA),prostaglandin endoperoxide synthase 2(PTGS2),estrogen receptor beta(ESR2),insulin(INS),caspase-3(CASP3),phosphatidylinositol 3-kinase regulatory subunit 1(PIK3R1),and peroxisome proliferator-activated receptor gamma(PPARG).A total of 6 core chemical components were obtaind by the"chemical composition-target"network combined with the comprehensive evaluation of drugability,including Formononetin,3,3'-Dimethylquercetin,Pinocembrin,Kumatakenin,3',7-Dihydroxy-4',6-Dimethoxyisoflavone,and Liquiritigenin.Molecular docking was used to verify that there was a strong interaction between the core targets and the core chemical components.Gene ontology(GO)functional enrichment analysis yielded 71 biological processes(BP),5 cellular components(CC),and 13 molecular functions(MF).Kyoto encyclopedia of genes and genomes(KEGG)signaling pathway enrichment analysis obtained 72 items,and the significantly enriched signaling pathways included lipid and atherosclerosis(hsa05417),vascular endothelial growth factor(VEGF,hsa04370),TNF(hsa04668),regulation of adipocyte lipolysis(hsa04923),adenosine monophosphate-activated protein kinase(AMPK,hsa04152),and cell apoptosis(hsa04210),mainly involving mechanisms such as vascular physiology,cell death,glycolipid metabolism,energy metabolism,and immune inflammation.Conclusion:Tiaogan Formula in preventing and treating coronary heart disease with Qi stagnation and blood stasis syndrome was closely related to the mechanisms of vascular remodeling,regulating cell death,regulating glucose and lipid metabolism,energy metabolism,and immune inflammation.
Keywords：coronary heart diseaseQi stagnation and blood stasis syndromeTiaogan Formulapsycho-cardiology medicineanxietydepression
Publication Date：2026-03-25
Online Publishing Date：2026-04-07(First online date of this platform, not the publication date of the document)
Pages：11( 801-811 )