Periostin Regulating p38MAPK/EL/HL Signaling Pathway Affects Cholesterol Reverse Transport
CAO Yanhong
LI Zhanhai
YANG Huiyu
Abstract：Objective:To investigate the effects of periostin on lipid metabolism and its regulatory mechanism on reverse cholesterol transport(RCT)in atherosclerotic(AS)mice.Methods:Fifty specific pathogen-free(SPF)grade ApoE-/-male mice were randomly divided into five groups:ND group,HD group,HD+AngⅡ group,HD+POSTN-mus-769+AngⅡ group,and HD+POSTN mus+AngⅡ group,with 10 mice in each group.Serum levels of total cholesterol(TC),triglyceride(TG),high-density lipoproteincholestero(HDL-C),and low-density lipoprotein cholesterol(LDL-C)were detected by enzyme-linked immunosorbent assay(ELISA);Oil red O staining was used to observe the pathological morphology of aorta and liver tissues;Western Blot was used to detect the expression of periostin,p-p38MAPK,endothelial lipase(EL),and hepatic lipase(HL)proteins in liver tissue;and reverse transcription polymerase chain reaction(RT-PCR)was used to detect the expression of periostin,p-p38MAPK,EL,and HL mRNA in liver tissue.Results:Compared with the ND group,the HD group exhibited abnormal lipid metabolism,characterized by a higher positive rate of lipid deposition in the aortic intima(P＜0.05),a higher positive rate of lipid deposition in liver tissue(P＜0.05),and increased expression of HL and EL proteins and mRNA in liver tissue(P＜0.05).Compared with the HD group,the HD+AngⅡ group showed more severe lipid metabolism abnormalities,increased positive rates of lipid deposition in both the aortic intima(P＜0.05)and liver tissue(P＜0.05),and upregulated expression of periostin,p-p38MAPK,EL,and HL proteins and mRNA in liver tissue(P＜0.05).Compared with the HD+AngⅡ group,the HD+POSTN-mus-769+AngⅡgroup exhibited attenuated lipid metabolism abnormalities,decreased positive rates of lipid deposition in both the aortic intima(P＜0.05)and liver tissue(P＜0.05),and downregulated expression of periostin,p-p38MAPK,EL,and HL proteins and mRNA in liver tissue(P＜0.05).In contrast,the HD+POSTN-mus+AngⅡ group displayed exacerbated lipid metabolism abnormalities,increased positive rates of lipid deposition in both the aortic intima(P＜0.05)and liver tissue(P＜0.05),and enhanced expression of periostin,p-p38MAPK,EL,and HL proteins and mRNA in liver tissue(P＜0.05).Conclusion:AngⅡ can induce periostin expression and inhibit RCT and promote AS progression by regulating the p38MAPK/EL/HL signaling pathway.
Keywords：atherosclerosisperiostinendothelial lipasehepatic lipasecholesterol reverse transportexperimental study
Publication Date：2026-02-25
Online Publishing Date：2026-03-16(First online date of this platform, not the publication date of the document)
Pages：9( 530-538 )