Mechanism of Huoxue Yixin Formula in the Treatment of Acute Coronary Syndrome Based on Network Pharmacology and Metabonomics
ZHANG Lei
FENG Juan
DU Mingyuan
SUN Yanqin
ZHU Cuiling
LIU Gang
LI Xingyuan
LI Yizhuo
YAN Haifeng
YAN Kuipo
DUAN Fei
DENG Daqing
YU Zaixin
Abstract：Objective:To explore the mechanism of Huoxue Yixin Formula in the treatment of acute coronary syndrome.Methods:The possible action pathways of Huoxue Yixin Formula in the treatment of acute coronary syndrome were predicted by network pharmacology.Non-targeted metabolomics analysis of serum samples from patients with acute coronary syndrome before and after treatment was conducted in combination with high-performance liquid chromatography-mass spectrometry technology,and pathway analysis of differential metabolites was performed.The results of network pharmacology and metabolomics were integrated and analyzed.Results:One hundred and seventy-two related pathways were preliminarily predicted by network pharmacology,fifty-four differential metabolites were detected by serum metabolomics,and seventy-six related pathways were obtained by differential metabolites pathway analysis.The mechanism of Huoxue Yixin Formula in the treatment of acute coronary syndrome may be related to phenylalanine metabolism,tyrosine metabolism,arginine and proline metabolism,pantothenate and CoA biosynthesis,adrenergic signaling pathway in cardiomyocytes,insulin resistance,hypoxia-inducible factor-1(HIF-1)signal pathway,and cyclic adenosine monophosphate(cAMP)signal pathway.Conclusion:This study systematically elaborated the mechanism of Huoxue Yixin Formula in the treatment of acute coronary syndrome from the aspects of gene and metabolism.It provided a certain reference for the development of follow-up trials and the research and development of new drugs.
Keywords：acute coronary syndromeHuoxue Yixin Formulanetwork pharmacologymetabonomicshigh performance liquid chromatography-mass spectrometry
Publication Date：2026-02-25
Online Publishing Date：2026-03-16(First online date of this platform, not the publication date of the document)
Pages：12( 481-492 )