The Mechanism of Xiaoke Yin on Metabolic Syndrome and Insulin Resistance Based on Network Pharmacology and Molecular Docking
LI Baoying
ZHA Yuling
DENG Mi
NIU Lu'na
LI Xuefei
ZHU Ruowei
JING Lu
Abstract：Objective:To explore the mechanism of Xiaoke Yin on the metabolic syndrome and insulin resistance based on network pharmacology and molecular docking.Methods:Based on network pharmacology methods and databases,Cytoscape was used to screen the core potential targets of Xiaoke Yin on metabolic syndrome and insulin resistance.The action pathways and target proteins were obtained through enrichment analysis.Based on the molecular docking method and database,the docking study of the active ingredients and pathway proteins of Xiaoke Yin was conducted to analyze the binding energy with the software of PyMOL and AutoDock.Results:A total of 30 potential core targets of Xiaoke Yin on the metabolic syndrome and insulin resistance were screened and obtained,including tumor necrosis factor(TNF),protein kinase B(AKT),peroxisome proliferator-activated receptor α(PPARα),hypoxia-inducible factor-1α(HIF-1α).Four pathways of action for Xiaoke Yin included respectively TNF/interleukin-6(IL-6),HIF-1α/heme oxygenase 1(HMOX1),AKT/mammalian target of rapamycin(mTOR),and PPARα/cholesterol 7α-hydroxylase(CYP7A1).The active ingredients of Xiaoke Yin could freely bind with pathway proteins to form hydrogen bonds,and the binding energy was relatively better.Conclusion:Xiaoke Yin could achieve the objective of metabolic syndrome and insulin resistance by regulating the TNF/IL-6,HIF-1α/HMOX1,AKT/mTOR,and PPARα/CYP7A1 pathways to downregulate inflammatory responses,inhibit ferroptosis,suppress cholesterol biosynthesis,and promote cholesterol metabolism.
Keywords：metabolic syndromeinsulin resistanceXiaoke Yinnetwork pharmacologymolecular dockingmechanism
Publication Date：2026-01-25
Online Publishing Date：2026-02-04(First online date of this platform, not the publication date of the document)
Pages：11( 186-196 )