The Effect of Nobiletin on TRPV1 Signaling Pathway in Regulating OGD/R-induced Apoptosis of Neural Cells
YU Man
WANG Fei
JIN Haitao
Abstract：Objective:To explore the effect of Nobiletin(NOB)on neuronal apoptosis induced by oxygen glucose deprivation/reoxygenation(OGD/R)by regulating the transient receptor potential vanilloid subfamily 1(TRPV1)signaling pathway.Methods:PC12 cells were cultured in primary culture and an OGD/R injury model was established.After treating OGD/R induced PC12 cells with 0,10,20,30,40,50,and 60 μmol/mL of NOB.Thiazolyl blue(MTT)assay was applied to detect cell survival rates in each group and screen for the optimal concentration of NOB.PC12 cells cultured in vitro were randomly separated into a control group(untreated),OGD/R group(OGD/R intervention in PC12 cells),OGD/R+NOB group(OGD/R+50 μmol/mL NOB co intervention in PC12 cells),and OGD/R+NOB+RTX(TRPV1 activator)group(OGD/R+50 μmol/mL NOB+10 nmol/L RTX co intervention in PC12 cells).MTT assay and flow cytometry were applied to detect the survival rate and apoptosis rate of cells in each group.Reagent kit method was applied to detect the levels of lactate dehydrogenase(LDH),malondialdehyde(MDA),superoxide dismutase(SOD),reduced glutathione(GSH),and tumor necrosis factor-α(TNF-α)in PC12 cells.Western Blot was applied to detect the expression of TRPV1,B lymphoblastoma-2(Bcl-2),and Bcl-2 associated X protein(Bax)proteins.Results:Compared with the control group,the survival rate,SOD,GSH levels,and Bcl-2 protein expression in PC12 cells in the OGD/R group were reduced(P＜0.05).Compared with the OGD/R group,the the survival rate,SOD,GSH levels,and Bcl-2 protein expression in the OGD/R+NOB group increased(P＜0.05).Compared with the OGD/R+NOB group,the survival rate,SOD,GSH levels,and Bcl-2 protein expression in the OGD/R+NOB+RTX group decreased(P＜0.05).Compared with the control group,the apoptosis rate,LDH,MDA,TNF-α levels,TRPV1,and Bax protein expression inPC12 cells in the OGD/R group increased(P＜0.05).Compared with the OGD/R group,the the apoptosis rate,LDH,MDA,TNF-α levels,TRPV1,and Bax protein expression inPC12 cells in the OGD/R+NOB group decreased(P＜0.05).Compared with the OGD/R+NOB group,the apoptosis rate,LDH,MDA,TNF-α levels,TRPV1,and Bax protein expression inPC12 cells in the OGD/R+NOB+RTX group increased(P＜0.05).Conclusion:NOB can reduce OGD/R induced inflammatory response and oxidative stress levels by inhibiting the activation of TRPV1 signaling pathway,ultimately alleviating OGD/R induced PC12 cell apoptosis.
Keywords：oxygen glucose deprivation/reoxygenationNobiletintransient receptor potential vanilloid subfamily 1neural cellsapoptosis
Publication Date：2026-01-10
Online Publishing Date：2026-01-16(First online date of this platform, not the publication date of the document)
Pages：6( 64-69 )