Study on the Mechanism of Seabuckthorn Flavone Inhibiting the p38MAPK/NF-κB Signaling Pathway to Improve Ischemic Arrhythmia in Rats
YANG Na
CAO Yueling
GUO Lihua
YUE Caiji
Abstract：Objective:To explore the effect of seabuckthorn flavone on ischemic arrhythmia in rats based on the p38 mitogen-activated protein kinase(p38MAPK)/nuclear factor-κB(NF-κB)signaling pathway.Methods:The rat model of ischemic arrhythmia was prepared by ligating the left anterior descending coronary artery.They were randomly divided into the model group,the low-dose seabuckthorn flavone group(100 mg/kg),the high-dose seabuckthorn flavone group(200 mg/kg),the propranolol group(15 mg/kg),and the seabuckthorn flavone+p38MAPK activator group(200 mg/kg+25 μg),with 12 rats in each group.Another 12 rats were taken as the sham operation group.Continuous intervention for 7 days.Twenty-four hours after the last administration,electrocardiogram was used to monitor the occurrence times of ventricular premature contractions(VPC),ventricular tachycardia(VT),and ventricular fibrillation(VF)within 30 minutes,and to evaluate the severity of arrhythmia.The biological signal acquisition system was used to detect changes in cardiac function indicators such as heart rate(HR),left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP),maximum rate of increase in left ventricular pressure(+dp/dtmax),and maximum rate of decrease in left ventricular pressure(-dp/dtmax)within 30 minutes.Hematoxylin-eosin staining was used to observe the histopathological changes of myocardial tissue in the ischemic area.The levels of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),and nerve growth factor(NGF)in myocardial tissues of the ischemic area were detected by enzyme-linked immunosorbent assay(ELISA).The expression of p38MAPK/NF-κB signaling pathway-related proteins in myocardial tissues of ischemic areas was detected by Western Blot.Results:Compared with the sham operation group,the myocardial fibers of rats in the model group were irregularly arranged,there was inflammatory cell infiltration in the myocardial interstitium,the stained myocardial fibers were distributed in a platelet-like manner,the electrocardiogram was abnormal,and the phenomenon of high T wave or ST segment elevation occurred,the occurrence frequencies of VPC,VT,VF,arrhythmia score,HR,LVEDP,IL-1β,TNF-α,NGF levels,p38MAPK,NF-κB protein expressions increased,while LVSP,+dp/dtmax,-dp/dtmax decreased(P＜0.05).Compared with the model group,in the low-dose seabuckthorn flavone group,the high-dose seabuckthorn flavone group,and the propranolol group,the infiltration of inflammatory cells in the myocardial tissue of the ischemic area in rats decreased,and the myocardial tissue injury,abnormal electrocardiogram conditions,and the phenomenon of high T wave or ST segment elevation improved,the occurrence frequencies of VPC,VT,VF,arrhythmia score,HR,LVEDP,IL-1β,TNF-α,NGF levels,p38MAPK,NF-κB protein expressions decreased,while LVSP,+dp/dtmax,-dp/dtmax increased(P＜0.05).The improvement effect of the above indicators in the high-dose seabuckthorn flavone group was better than that in the low-dose seabuckthorn flavone group(P＜0.05).There was no statistically significant difference in the above indicators between the high-dose seabuckthorn flavone group and the propranolol group(P＞0.05).Compared with the high-dose seabuckthorn flavone group,the degree of myocardial tissue injury and inflammatory cell infiltration in the ischemic area of rats in the seabuckthorn flavone+p38MAPK activator group were aggravated,the electrocardiogram was significantly abnormal,and the phenomenon of high T wave or ST segment elevation was aggravated,the occurrence frequencies of VPC,VT,VF,arrhythmia score,HR,LVEDP,IL-1β,TNF-α,NGF levels,p38MAPK,NF-κB protein expressions increased,while LVSP,+dp/dtmax,-dp/dtmax decreased(P＜0.05).Conclusion:Seabuckthorn flavone can improve the symptoms,myocardial tissue injury in the ischemic area and cardiac function in rats with ischemic arrhythmia,and inhibit the levels of inflammatory factors and NGF,which may be related to its inhibition of the activity of the p38MAPK/NF-κB signaling pathway.
Keywords：ischemic arrhythmiaseabuckthorn flavonep38 mitogen-activated protein kinasenuclear factor-κBexperimental study
Publication Date：2026-01-10
Online Publishing Date：2026-01-16(First online date of this platform, not the publication date of the document)
Pages：7( 50-56 )