The Effects of Atractylodes-Ephedra on the Lipid Levels and Fat Metabolism in Zebrafish with Dyslipidemia
HU Xin
SHANG Juju
LIU Hongxu
ZHOU Mingxue
LI Sinai
LI Lulu
Abstract：Objective:To explore the effects of Atractylodes-Ephedra on lipid levels and fat metabolism in zebrafish with dyslipidemia.Methods:The albino zebrafish strain with melanin allele mutation that semi-transparent was naturally mated in pairs for reproduction.A total of 210 fish(30 fish per group)were selected at 7 days post-fertilization(7dpf)for evaluating the lipid-lowering effect of traditional Chinese medicine decoctions.The albino zebrafish strain with melanin allele mutation that isemi-transparent was naturally mated in pairs for reproduction.Five days post-fertilization(5dpf),210 fish were selected(30 fish per group),for evaluating the effect of traditional Chinese medicine decoctions on adipose catabolism.Results:Compared with the model control group,in the simvastatin group and the 25.0,50.0,and 100.0 μL/mL concentrations of the Atractylodes-Ephedra formula groups,the sum of optical density were statistically significantly different(P＜0.05).It was demonstrated that at 40 ng/mL simvastatin and 25.0,50.0,and 100.0 μL/mL of Atractylodes-Ephedra Formula had lipid-lowering effects on zebrafish with dyslipidemia induced by egg yolk powder.Compared with the model control group,in the simvastatin group and the 25.0,50.0,and 100.0 μL/mL concentrations of the Atractylodes-Ephedra Formula,the TG levels showed statistically significant differences(P＜0.05).It was demonstrated that at concentrations of 40 ng/mL simvastatin and 25.0,50.0,and 100.0 μL/mL of Atractylodes-Ephedra Formula,the TG levels in zebrafish with dyslipidemia induced by egg yolk powder were reduced.Compared with the model control group,in the simvastatin group and the 12.5,25.0,50.0,and 100.0 μL/mL concentrations of Atractylodes-Ephedra Formula group,the TC levels showed statistically significant differences(P＜0.05).It was shown that at concentrations of simvastatin of 40 ng/mL and of 12.5,25.0,50.0,and 100.0 μL/mL,the concentration of Atractylodes-Ephedra Formula could reduce the TC level of the zebrafish with dyslipidemia induced by egg yolk powder.Compared with the positive control group,there were statistically significant differences in the total staining intensity of the yolk sac and adipose catabolism in the resveratrol group and the 100.0 μL/mL concentration group of the Atractylodes-Ephedra Formula(P＜0.05).It was shown that at a concentration of 14.4 μg/mL of resveratrol and 100.0 μL/mL of Atractylodes-Ephedra Formula,there was a promoting effect on the adipose catabolism of zebrafish.Conclusion:Atractylodes-Ephedra herbs have the effect of reducing triglyceride(TG)and total cholesterol(TC)levels and promoting adipose catabolism.
Keywords：dyslipidemiaAtractylodesEphedrafat metabolismzebrafish
Publication Date：2026-01-10
Online Publishing Date：2026-01-16(First online date of this platform, not the publication date of the document)
Pages：5( 45-49 )