Effect of Andrographolide on Myocardial Injury in Rats with Acute Myocardial Infarction via Regulation of the IL-6/STAT3 Signaling Pathway
MA Weiqian
LIU Ming
WEI Juan
ZHANG Yunqing
YAN Yuejuan
Abstract：Objective:To investigate the effect of andrographolide(Andro)on myocardial injury in rats with acute myocardial infarction(AMI).Methods:Rat AMI model was constructed by ligating the left anterior descending coronary artery(LAD),and the rats were randomly assigned to the sham surgery group,model group,low-,medium-,and high-dose Andro groups,and Colivelin group.The cardiac function of rats was tested;2,3,5-triphenyltetrazolium chloride(TTC)staining was applied to observe the condition of myocardial infarction.Hematoxylin and eosin(HE)staining was used to examine pathological changes in myocardial tissue.The assay kits were used to detect lactate dehydrogenase(LDH),creatine kinase isoenzyme(CK-MB),cardiac troponin I(cTnI),interleukin-1 β(IL-1 β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α).Western Blot was performed to detect the expression levels of IL-6,phosphorylated signal transducer and activator of transcription 3(p-STAT3),and ignal transducer and activator of transcription 3(STAT3)proteins in myocardial tissue.Results:Compared with the sham surgery group,the myocardial tissue damage in the model group was severe,the LVEDD,LVESD,LDH,infarct size,CK-MB,cTnI,TNF-α,IL-1 β,IL-6,myocardial cell apoptosis rate,IL-6,p-STAT3/STAT3 significantly increased,the LVFS and LVEF significantly decreased(P＜0.05);Compared with the sham surgery group,the myocardial tissue damage in the model group was more severe;left ventricular end-systolic diameter(LVESD),left ventricular end-diastolic diameter(LVEDD),LDH,infarct size,CK-MB,cTnI,TNF-α,IL-1 β,IL-6,myocardial cell apoptosis rate,and p-STAT3/STAT3 significantly increased,while left ventricular short-axis ejection fraction(LVEF),short-axis shortening rate(LVFS)significantly decreased(P＜0.05).Compared with the model group,the myocardial injury in the low-,medium-,and high-dose Andro groups improved;the LVEDD,LVESD,LDH,infarct size,CK-MB,cTnI,TNF-α,IL-1β,IL-6,myocardial cell apoptosis rate,and p-STAT3/STAT3 significantly decreased,while the LVFS and LVEF significantly increased(P＜0.05).The STAT3 activator Colivelin reversed the protective effects of Andro on myocardial injury.Conclusion:Andro can improve myocardial injury,improve cardiac function,reduce myocardial infarction,inflammatory response,and cell apoptosis in AMI rats,and its action may relate the inhibition of the IL-6/STAT3 pathway.
Keywords：acute myocardial infarctionmyocardial injuryandrographolideinterleukin-6signal transducers and activators of transcription-3experimental study
Publication Date：2025-12-25
Online Publishing Date：2026-01-13(First online date of this platform, not the publication date of the document)
Pages：7( 3739-3745 )