Effects of Ethyl Acetate Extract of Gentiana Mahua on Myocardial Injury,Mitochondrial Energy Metabolism,and Nrf2,HO-1,and NQO1 Proteins Expression in Myocardial Ischemia-reperfusion Rats
WANG Rong
LIU Mingchen
SONG Lijuan
HAN Yi
Abstract：Objective:To investigate the effects of ethyl acetate extract of Gentiana mahua on myocardial injury,mitochondrial energy metabolism,and the expression of nuclear factor erythroid 2-related factor(Nrf2),haem oxygenase-1(HO-1),and quinone oxidoreductase 1(NQO1)proteins in rats with myocardial ischemia-reperfusion injury(MIRI).Methods:Fifty rats were randomly divided into sham operation group,model group,positive control group,low-dose group,and high-dose group,with 10 rats in each group.Except for the sham operation group,MIRI rat models were established in the other four groups.The rats in the positive control group were given 19.64 mg/kg suspension of propranolol hydrochloride,the rats in the low-dose and high-dose groups were administered 3.27 g/kg and 6.55 g/kg of ethyl acetate extract of gentiana Mahua,respectively,and the rats in the sham operation group and the model group were given an equivalent volume of normal saline.Terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL)staining was used to observe the apoptosis rate in each group.Triphenyltetrazolium chloride(TTC)was used to determine the myocardial infarction volume.The changes in mitochondrial ultrastructure and energy metabolism,as well as the activities of mitochondrial ATPase,Na+-K+-ATPase,and Ca2+-Mg2+-ATPase,were observed by electron microscopy.The morphology of myocardium was observed by hematoxylin-eosin(HE)staining.The expression levels of Nrf2,HO-1,and NQO1 proteins in myocardial tissue were detected by Western Blotting.Results:Compared with sham operation group,the apoptosis rate and percentage of myocardial infarction volume of rats in all modeling groups increased,and the protein expression levels of ATPase,Na+-K+-ATPase,Ca2+-Mg2+-ATPase,Nrf2,HO-1,and NQO1 decreased(P＜0.05).The percentages of myocardial apoptosis and myocardial infarction volume in the positive control group and the low-and high-dose groups decreased,and the high-dose group was lower than both the positive control group and the low-dose group(P＜0.05).The protein expression levels of ATPase,Na+-K+-ATPase,Ca2+-Mg2+-ATPase,Nrf2,HO-1,and NQO1 increased,and the high-dose group was higher than both the positive control group and the low-dose group(P＜0.05).Among the modeling groups,the structure of cardiomyocytes in the high-dose group was the best.Conclusion:Ethyl acetate extract of Gentiana mavarii can effectively reduce myocardial injury,regulate mitochondrial energy metabolism,and enhance Nrf2,HO-1,and NQO1 protein expression in the myocardium of MIRI rats.
Keywords：myocardial ischemia-reperfusionethyl acetate extract of gentiana Mahuamyocardial damagemitochondrial energy metabolismnuclear factor erythroid 2-related factorexperimental study
Publication Date：2025-12-10
Online Publishing Date：2025-12-19(First online date of this platform, not the publication date of the document)
Pages：7( 3577-3583 )