The Effect of Diosmetin on H/R-induced Cardiomyocyte Injury by Regulating the RhoA/ROCK Signaling Pathway
ZHANG Rui
ZHAO Ningyi
LIAO Qiang
GENG Jianglun
Abstract：Objective: To observe the effect of dihydroquercetin (Dio) regulating the RhoA/Rho-associated coiled-coil containing protein kinase (ROCK) signaling pathway on myocardial cell injury induced by hypoxia/reoxygenation (H/R). Methods: H9c2 cells were randomly divided into blank group (normal H9c2 cells cultured under routine conditions), H/R group (H9c2 cells treated with H/R), L-Dio group (5 μg/mL Dio + H/R-treated H9c2 cells), M-Dio group (10 μg/mL Dio + H/R-treated H9c2 cells), H-Dio group (15 μg/mL Dio + H/R-treated H9c2 cells), and LPA group (15 μg/mL Dio + 10 μg/g RhoA pathway activator LPA + H/R-treated H9c2 cells). All cells were cultured in routine medium for 48 hours. The survival rate, apoptosis rate, and autophagy status of each group were observed. The activities of superoxide dismutase (SOD), levels of malondialdehyde (MDA) and reactive oxygen species (ROS), as well as the expression of RhoA/ROCK pathway-related, autophagy-related, and apoptosis-related proteins were measured. Results: Compared with the blank group, the H/R group showed increased apoptosis rate, number of autophagosomes and autolysosomes, expression of RhoA, ROCK1, ROCK2, LC3B, Bax, Caspase-3 proteins, and levels of MDA and ROS (P < 0.05), while the survival rate, P62 protein expression, and SOD activity decreased (P < 0.05). Compared with the H/R group, the L-Dio, M-Dio, and H-Dio groups showed increasing survival rate, P62 protein expression, and SOD activity, and decreasing apoptosis rate, number of autophagosomes and autolysosomes, expression of RhoA, ROCK1, ROCK2, LC3B, Bax, Caspase-3 proteins, and levels of MDA and ROS (P < 0.05). Compared with the H-Dio group, the LPA group showed increased apoptosis rate, number of autophagosomes and autolysosomes, expression of RhoA, ROCK1, ROCK2, LC3B, Bax, Caspase-3 proteins, and levels of MDA and ROS (P < 0.05), while the survival rate, P62 protein expression, and SOD activity decreased (P < 0.05). Conclusion: Dio may inhibit H/R-induced myocardial cell injury by inhibiting the RhoA/ROCK pathway, regulating apoptosis and autophagy.
Keywords：ischemic heart diseasecardiomyocyte injuryH9c2 cellsdiosmetinRas homolog gene family member A/RHO-associated kinase signaling pathwayexperimental study
Publication Date：2025-11-25
Online Publishing Date：2025-12-05(First online date of this platform, not the publication date of the document)
Pages：7( 3432-3438 )