The Mechanism of Quanzhen Yiqi Decoction for the Treatment of Chronic Heart Failure Based on Network Pharmacology and Basic Experiment
YE Jingxian
LU Jianqi
HUANG Xiaoyu
LI Honghui
TAN Wei
WEN Zhihao
Abstract：Objective:To explore the target and mechanism of action of Quanzhen Yiqi Decoction for the treatment of chronic heart failure(CHF)based on network pharmacology and animal experiments.Methods:The action targets of Quanzhen Yiqi Decoction were collected through network pharmacology and basic retrieval.The relevant target information of Quanzhen Yiqi Decoction was collected and analyzed from the TCMSP and ETCM databases of the System Pharmacology Platform of Traditional Chinese medicine.The information of CHF targets was obtained through the GeneCards and DisGeNET databases.Pathway enrichment analyses were conducted through the DAVID database for Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG).Molecular docking verification was conducted in the AutoDock software.The pathological changes of cardiac tissue were observed by hematoxylin-eosin(HE)staining.Western Blot was used to verify the possible targets screened out by network pharmacology.The indicators of serum-related heart failure and inflammation in CHF rats were detected by enzyme-linked immunosorbent assay(ELISA).Results:A total of 86 effective active ingredients and 634 predicted action targets of Quanzhen Yiqi Decoction were collected by network pharmacology.Western Blot verified that the target proteins,including mitogen-activated protein kinase 14(MAPK14),protein kinase B1(AKT1),tumor necrosis factor(TNF)-α,nuclear transcription factor κB(NF-κB),and other key factors played some regulatory roles on CHF.Conclusion:Network pharmacology and basic experiments showed that Quanzhen Yiqi Decoction might treat CHF by influencing core proteins such as MAPK14,AKT1,TNF-α,and NF-κB,thereby regulating the phosphoinositide 3-kinase/protein kinase B(PI3K/AKT)signaling pathway,the mitogen-activated protein kinase(MAPK)signaling pathway,and the TNF signaling pathway.
Keywords：chronic heart failureQuanzhen Yiqi Decoctionnetwork pharmacologytargetmechanism of actionbasic experiment
Publication Date：2025-11-25
Online Publishing Date：2025-12-05(First online date of this platform, not the publication date of the document)
Pages：11( 3405-3415 )