Effect of Sequoiol on H2O2 Induced Myocardial Cell Injury by Regulating the Hippo Signaling Pathway
WU Liguo
MA Rong
YAN Guangyou
GENG Xuebin
Abstract：Objective:To explore the effect of Sequoiol on H2O2 induced myocardial cell injury by regulating the Hippo signaling pathway.Methods:Human myocardial cells AC16 were divided into the control group,H2O2 group,low,medium,and high-dose Sequoiol groups(L-Sequoiol group,M-Sequoiol group,H-Sequoiol group),and XMU-MP-1 group.EdU staining was applied to detect AC16 cell proliferation.The apoptosis of AC16 cells was detected by the Hoechst 33258 staining method.γ-H2AX immunofluorescence assay was applied to detect the level of DNA damage in AC16 cells.The enzyme-linked immunosorbent assay(ELISA)method was used to detect the levels of superoxide dismutase(SOD)and reactive oxygen species(ROS)in the serum of AC16 cells.The protein immunoblotting method(Western Blot)was used to detect the protein expressions of YAP1,p-YAP1,TAZ,γ-H2AX,B-cell lymphoma 2(Bcl-2),Bcl-related X protein(Bax),and Caspase-3 in AC16 cells.Results:Compared with the control group,the EdU positive cell rate,SOD,and p-YAP1,Bcl-2 protein expression of AC16 cells in the H2O2 group decreased,the apoptosis rate,DNA damage,ROS,YAP1,TAZ and γ-H2AX,Bax,and Caspase-3 protein expression levels increased(P＜0.05);compared with the H2O2 group,the EdU positive cell rate,p-YAP1,SOD,and Bcl-2 protein expression increased in the L-Sequoiol group,M-Sequoiol group,and H-Sequoiol group,the apoptosis rate,DNA damage,ROS and YAP1,TAZ,γ-H2AX,Bax,Caspase-3 protein expression levels decreased,and as the concentration of Sequoiol increased,with a gradually increasing or decreasing trend(P＜0.05);the EdU positive cell rate,SOD and p-YAP1,Bcl-2 in the XMU-MP-1 group were lower than those in the H-Sequoiol group,the apoptosis rate,DNA damage,ROS and YAP1,TAZ,γ-H2AX,Bax,Caspase-3 protein expression in the H-Sequoiol group were higher than those in the H-Sequoiol group(P＜0.05).Conclusion:Sequoiol can inhibit H2O2 induced myocardial cell injury,and its mechanism relates regulating the Hippo signaling pathway.
Keywords：myocardial cell injurySequoiolHippo signaling pathwayH2O2experimental study
Publication Date：2025-11-10
Online Publishing Date：2025-11-24(First online date of this platform, not the publication date of the document)
Pages：8( 3247-3254 )