The Molecular Mechanism of Dendrobium Officinale in Anti-atherosclerosis Based on Mitochondrial Autophagy-NLRP3 Inflammatory Body
SHI Yujie
LIU Xiaoli
HAN Juncheng
SHI Yuqin
Abstract：Objective:To explore the molecular mechanism of Dendrobium officinale in anti-atherosclerosis(AS)based on mitochondrial autophagy-nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)inflammatory body.Methods:Six C57BL/6J mice were selected as the control group.Eighteen ApoE-knockout(ApoE-/-)mice were randomly divided into the AS group,Dendrobium officinale group,and Dendrobium officinale+mitochondrial fission inhibitor-1(Mdivi-1)group,with 6 mice in each group.The levels of total cholesterol(TC),triglycerides(TG),and low-density lipoprotein(LDL)in the serum of each group of mice were measured.Hematoxylin-eosin(HE)staining was used to detect the pathological structure of the thoracic aorta in mice.Oil Red O staining was used to detect lipid infiltration in the thoracic aorta of mice.Western Blot was used to detect the levels of mitochondrial autophagy-related protein Beclin1,autophagy microtubule-associated protein light chain 3 Ⅱ(LC3 Ⅱ),and NLRP3 inflammasome protein.Real-time fluorescent quantitative polymerase chain reaction(RT-qPCR)was used to detect the mRNA levels of Beclin1,LC3 Ⅱ and NLRP3 inflammasome.Immunohistochemical staining was used to detect the level of NLRP3 protein in the thoracic aorta of mice.The levels of inflammatory factors such as tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-18(IL-18),and interleukin-1β(IL-1β)in the serum of each group of mice were detected.Results:Compared with the AS group,the levels of TC,TG,and LDL in the serum of mice in the Dendrobium officinale group decreased(P＜0.05).The HE staining results showed that compared with the control group,the intima of the thoracic aorta in the AS group was thickened and disordered,with plaque formation observed;compared with the AS group,the structure of the thoracic aorta in the Dendrobium officinale group improved significantly.The oil red O staining results showed that,compared with the control group,a large amount of lipid infiltration could be observed in the thoracic aorta of the AS group mice;and compared with the AS group,the lipid infiltration in the thoracic aorta of the Dendrobium officinale group mice was improved.Compared with the control group,the expression of Beclin1,LC3Ⅱ protein and mRNA in the thoracic aorta of the AS group decreased(P＜0.05);compared with the AS group,the expression of Beclin1,LC3Ⅱ protein and mRNA in the thoracic aorta of the Dendrobium officinale group increased(P＜0.05).Compared with the control group,the levels of TC,TG and LDL in the AS group decreased(P＜0.05),the expression of NLRP3 protein and mRNA increased(P＜0.05),and the levels of TNF-α,IL-6,IL-18 and IL-1β in the serum increased(P＜0.05).Compared with the AS group,the levels of TC,TG and LDL in the Dendrobium officinale group increased(P＜0.05),the expression of NLRP3 protein and mRNA decreased(P＜0.05),and the levels of TNF-α,IL-6,IL-18 and IL-1β in the serum decreased(P＜0.05).Compared with the Dendrobium officinale group,the serum levels of TC,TG and LDL in Dendrobium officinale+Mdivi-1 group increased(P＜0.05),the expression of NLRP3 protein and mRNA increased(P＜0.05),and the levels of TNF-α,IL-6,IL-18 and IL-1β increased(P＜0.05).Conclusion:Dendrobium officinale can improve atherosclerosis by up-regulating mitophagy and down-regulating NLRP3-mediated inflammatory response.
Keywords：atherosclerosisDendrobium officinalemitochondrial autophagynucleotide-binding oligomerization domain-like receptor protein 3blood lipidsinflammatory factorsexperimental study
Publication Date：2025-10-10
Online Publishing Date：2025-10-24(First online date of this platform, not the publication date of the document)
Pages：7( 2942-2948 )