The Mechanism of Action of Shexiang Tongxin Dropping Pills in Treating Coronary Heart Disease with Phlegm-heat and Blood Stasis Syndrome Based on the NF-κB Signaling Pathway
HUANG Xing
KOU Guanjun
ZHANG Zhiguo
Abstract：Objective:To explore the mechanism of action of Shexiang Tongxin Dropping Pills in treating coronary heart disease with phlegm-heat and blood stasis syndrome based on the nuclear factor-κB(NF-κB)signaling pathway-related factors.Methods:Sixty-nine male specific-pathogen-free(SPF)grade SD rats were acclimated for 2 weeks.Nine rats were selected as the control group,while the remaining 60 rats were induced to develop the coronary heart disease model of phlegm-heat and blood stasis syndrome through drug and surgical methods.In the sham operation group,there were 9 rats.The remaining rats that underwent coronary artery ligation were successfully operated and survived.These rats were randomly divided into the model group,the low-dose group of Shexiang Tongxin Dropping Pills,the medium-dose group of Shexiang Tongxin Dropping Pills,and the high-dose group of Shexiang Tongxin Dropping Pills.The drug intervention lasted for 4 weeks.The general conditions of the rats,including body weight,activity status,defecation and urination,and hair condition.The changes of ECG in rats before and after coronary artery ligation and at the end of the experiment were recorded.The levels of serum low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),triglycerides(TG),and total cholesterol(TC)were measured.The levels of serum interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and NF-κB were detected by enzyme-linked immunosorbent assay(ELISA).Results:Compared with the model group,the TC,TG,LDL-C,IL-1β,IL-6,and TNF-α levels in the low-dose,medium-dose,and high-dose groups of Shexiang Tongxin Dropping Pills decreased(P＜0.05),while the HDL-C level increased(P＜0.05).The levels of TC,TG,LDL-C,IL-1β and NF-κB in the medium-dose and the high-dose Shexiang Tongxin Dropping Pills groups were significantly lower than those in the low-dose group(P＜0.05).The levels of TG and LDL-C in the high-dose Shexiang Tongxin Dropping Pills group were lower than those in the medium-dose group(P＜0.05).Conclusion:Shexiang Tongxin Dropping Pills show a certain protective effect on coronary heart disease rats with phlegm-heat and blood stasis syndrome,reducing myocardial cell apoptosis.The mechanism of action may be related to the regulation of inflammatory factors IL-1β,IL-6,TNF-α levels and the expression of NF-κB protein.
Keywords：coronary heart diseasesyndrome of phlegm-heat and blood stasisShexiang Tongxin Dropping Pillsnuclear factor-κB signaling pathwayblood lipidsinflammatory factorsexperimental study
Publication Date：2025-10-10
Online Publishing Date：2025-10-24(First online date of this platform, not the publication date of the document)
Pages：5( 2937-2941 )