Mechan ism of Banxia Baizhu Tianma Decoction Combined with Aspirin in the Treatment of TIA Based on Network Pharmacology and Experimental Studies
LI Hongyang
QIU Jinling
YANG Ruo
LING Jie
ZHANG Yueyang
GU Wei
Abstract：Objective:To explore effect of Banxia Baizhu Tianma Decoction and aspirin has a better effect on the neurological function and ischemic lesions of rats with transient ischemic attack(TIA)models,and to investigate the material basis and mechanism of action of the treatment of TIA with Banxia Baizhu Tianma Decoction combined with aspirin based on network pharmacology and molecular docking.Methods:TIA model rats were prepared using longa method,and were randomly divided into the model group,the sham operation group,the western medicine group(aspirin group),the traditional Chinese medicine group(Banxia Baizhu Tianma Decoction group),and the treatment group(Banxia Baizhu Tianma Decoction combined with aspirin group).The rats were treated by intragastric administration for 7 days.The neurological function scoring was tested before and after drug administration for each group of animals.The chemical components and targets of the traditional Chinese medicine,Banxia Baizhu Tianma Decoction were retrieved and screened through the traditional Chinese medicine system pharmacology analysis platform,PubChem and PharmMapper databases.The chemical components of aspirin were searched using the keyword"aspirin"from GeneCards database.The relevant target genes related to TIA retvieved from the DisGeNET online database.The common targets between drugs and diseases were obtained using Venny 2.1 software.Protein interaction network analysis was conducted through the STRING 10.5 database.The drug-component-target-disease network was constructed using Cytoscape 3.7.1 software.Gene ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed using the DAVID 6.8 online database.Finally,the binding ability of the core components and the core targets was verified through molecular docking.Results:Animal experiments showed that the treatment group could significantly improve the neurological function scores of TIA model rats and significantly reduce the area of ischemic lesions.Quercetin,aspirin,kaempferol,naringin,β-sitosterol and atractylaride Ⅲ might be the key active substances in the combination of Banxia Baizhu Tianma Decoction and aspirin for the treatment of TIA.Interleukin 6(IL6),tumor necrosis factor(TNF),interleukin 1β(IL1B),albumin(ALB),vascular endothelial growth factor A(VEGFA),etc.,might be the main intervention targets.Blood lipids,arterial atherosclerosis,the promotion of atherosclerosis by blood flow shear stress,platelet activation channels,tumor necrosis factor signaling pathway,mitogen-activated protein kinase(MAPK)signaling pathway,and interleukin-17(IL-17)signal transduction were all key influencing pathways.Molecular docking studies have showed that the aforementioned main components exhibited excellent binding capabilities with the target.Conclusion:Banxia Baizhu Tianma Decoction and aspirin for the treatment of TIA show the characteristics of systemic multi-target and multi-pathway effects,and the potential molecular mechanisms are preliminarily explained.
Keywords：transient ischemic attacksBanxia Baizhu Tianma Decoctionaspirinnetwork pharmacologymolecular docking
Publication Date：2025-10-10
Online Publishing Date：2025-10-24(First online date of this platform, not the publication date of the document)
Pages：11( 2926-2936 )