Molecular Mechanism of Yiqi Huoxue Tongmai Formula for the Treatment of Reverse-dipper Hypertension Based on Network Pharmacology
MIAO Huihui
LIU Zhongyong
TANG Nana
XU Yuanyuan
Abstract：Objective:To explore the potential mechanism of Yiqi Huoxue Tongmai Formula for the treatment of reverse-dipper hypertension based on network pharmacology.Methods:The bioactive components of Yiqi Huoxue Tongmai Formula were screened and their targets were predicted by using the Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),BATMAN-TCM Database,PubChem database and Swiss Target Prediction database.The DisGeNET,GeneCards and OMIM databases were applied to collect the relevant targets of reverse-dipper hypertension.The Venny 2.1 software was used to map the targets of the Yiqi Huoxue Tongmai Formula and reverse-dipper hypertension,obtain the common targets of drugs and diseases,draw the Venny diagram,construct the"drug-component-intersection target"network diagram,and to obtain the core active ingredients.The protein-protein interaction(PPI)network was constructed using the STRING database and Cytoscape 3.9.1 software to screen and obtain the core targets.The DAVID database was used for gene ontology(GO)functional annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis of common target genes for drug-disease.Results:A total of 109 compounds and 1 256 targets were screened out from the Yiqi Huoxue Tongmai Formula,including 1 231 targets for reverse-dipper hypertension,313 intersection targets and 11 key targets.The results of GO enrichment analysis showed there were 1 202 biological processes,125 cellular components,and 225 molecular functions.KEGG enrichment analysis included 189 signaling pathways.The potential targets involved in the intervention of the Yiqi Huoxue Tongmai Formula for reverse-dipper hypertension included insulin(INS),interleukin-6(IL6),tumour protein 53(TP53),transcription activator 3(STAT3),and tyrosine kinase(SRC).The related signaling pathways included the advanced glycation end products and their receptors(AGE-RAGE)signaling pathway in diabetic complications,the hypoxia-inducible factor 1(HIF-1)signaling pathway,the calcium signaling pathway,the phosphatidylinositol 3-kinase-protein kinase B(PI3K-AKT)signaling pathway,the mitogen-activated protein kinase(MAPK)signaling pathway,Rap1 signaling pathways,prolactin signaling pathways,and relaxin signaling pathways.Conclusion:Yiqi Huoxue Tongmai Formula for treating reverse-dipper hypertension showed the characteristics and advantages of multiple components,multiple targets and multiple pathways,and it could serve as an important research basis for further exploring the active substances and mechanism of action of Yiqi Huoxue Tongmai Formula in treating reverse-dipper hypertension.
Keywords：reverse-dipper hypertensionYiqi Huoxue Tongmai Formulanetwork pharmacologytargetmolecular mechanism
Publication Date：2025-09-25
Online Publishing Date：2025-10-14(First online date of this platform, not the publication date of the document)
Pages：12( 2721-2732 )