Exploration of the Epigenetic Characteristics of the Taiyin Type Patients with Cerebral Infarction Based on the Differences of DNA Methylation
LIU Xiangzhe
WANG Xiaoli
LU Yongkun
YANG Haiyan
ZHOU Hongxia
WANG Xinzhi
Abstract：Objective:To explore the differences of DNA methylation and the epigenetic characteristics of the Taiyin type patients with cerebral infarction,and to provide theoretical basis for the prevention and treatment of cerebral infarction.Methods:Based on the theory of"Five-State Personality"in traditional Chinese medicine and according to the"Five-State Personality Test Form(Short Version for Adults)",Taiyin-type patients with cerebral infarction were selected as the research subjects,and Yin-Yang peace type patients were selected as controls.The patient's whole blood specimens were collected and stored at-80℃.Illumina 850k DNA methylation chip sequencing method was used to determine gene differential methylation sites.Candidate genes relevant to the study were screened by significance of difference,gene ontology(GO),and Kyoto Encyclopedia of Genes and Genomes(KEGG)annotations.Results:Based on Illumina 850K chip detection analysis,there were 346 differential methylation sites and 189 genes among the Taiyin type and the Yin-Yang peace type patients,including 175 low methylation sites and 171 high methylation sites.There were 101 genes with highly methylated sites and 94 genes with lowly methylated sites.Among them,CHKB-CPT1B,LOC101927502,FAM66D,TBCD,GDF7,PTPRN2,PSORS1C1,C22orf34,KCNE1,FAM71F1,POLE,PIGG,KCNMA1,LOC285830,and HIF3A with multiple differentially methylated sites.The top 25 hypermethylated genes and top 25 hypomethylated genes annotated by GO analysis were ranked according to the absolute value of Delta_Beta.Among the above 50 differentially methylated genes,the hypomethylated genes in the promoter region were OR8A1 and OR2A5,and the hypermethylated genes were TP73,NUP35,TMEM9,CLPTM1,CPLX2,ACE,and B3GALT1.The differences in methylation sites between Taiyin type and Yin-Yang peace type patients mainly related to nutrient metabolism,ion transport,inflammation and immune response,neurotransmitter secretion,signal transduction,and the regulation of gene transcription and expression,etc.,which might be the inherent risk factors related incidence of cerebral infarction in Taiyin type patients.Conclusions:There is a significant difference in DNA methylation levels between Taiyin type and Yin-Yang peace type patients with cerebral infarction,which may relate to the difference in the incidence of cerebral infarction between the two groups.
Keywords：cerebral infarctionTaiyin typeDNA methylationepigenetics
Publication Date：2025-08-25
Online Publishing Date：2025-09-10(First online date of this platform, not the publication date of the document)
Pages：7( 2444-2450 )