Effect of Geniposide on Endoplasmic Reticulum Stress in Coronary Heart Disease-related Atherosclerosis through the SIRT1 Pathway
WANG Jiayi
HAN Ruiwei
HE Li
LIU Hui
Abstract：Objective:To analyze the effect of geniposide(GE)on endoplasmic reticulum stress(ERs)in coronary heart disease atherosclerosis through silent information regulator 1(SIRT1)pathway.Methods:Vascular smooth muscle cells(VSMCs)were divided into control group(Con),angiotensin(AngⅡ)group,GE group,and AngⅡ+GE group.The expressions of Cleaved Caspase-3,X-box binding protein 1(XBP-1),activating transcription factor 6(ATF-6),and ATF-6β proteins in VSMCs cells were analyzed by Western Blot.Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)staining was used to detect apoptosis,and immunofluorescence was used to detect SIRT1 expression.Thirty-two male ApoE-/-mice were randomly divided into four groups(8 mice in each group):control group(Ctrl),AngⅡ group,GE group,and AngⅡ+GE group.The mice in GE group and AngⅡ+GE group were given GE by gavage every day.At the end of the experimental period,aortic tissue was obtained from mice for analysis of apoptosis and ERs.Results:Compared with the Con group,the expression of cleaved Caspase-3,XBP1,and ATF-6β in the Ang Ⅱ group up-regulated(P＜0.05),and the percentage of TUNEL-positive cells increased significantly(P＜0.05).Compared with the AngⅡ group,the expression of Caspase-3,XBP1,and ATF-6β down-regulated(P＜0.05),and the percentage of TUNEL-positive cells decreased significantly(P＜0.05).In addition,the fluorescence intensity of SIRT1 increased significantly(P＜0.05)in the AngⅡ+GE group.Compared with the Ctrl group,the expression of cleaved caspase-3,XBP1,and ATF-6β proteins in the aortic intima of the AngⅡ group up-regulated(P＜0.05),and the percentage of TUNEL-positive tissues increased significantly(P＜0.05).Compared with the AngⅡ group,the expression levels of Caspase-3,XBP1,and ATF-6β proteins in the AngⅡ+GE group down-regulated(P＜0.05),and the percentage of TUNEL-positive tissues decreased significantly(P＜0.05).Compared with the AngⅡ group,the fluorescence intensity of SIRT1 in the AngⅡ+GE group increased significantly(P＜0.05).Conclusion:GE enhances the intervention of ERs in coronary atherosclerosis by changing SIRT1 signaling pathway,thus protecting VSMCs from apoptosis induced by AngⅡ.
Keywords：coronary heart diseaseatherosclerosisgeniposidesilent information regulator 1 pathwayendoplasmic reticulum stressvascular smooth muscle cellsexperiment study
Publication Date：2025-08-10
Online Publishing Date：2025-08-20(First online date of this platform, not the publication date of the document)
Pages：7( 2291-2297 )