The Effect of Fuling Wenshen Capsule on Ventricular Remodeling in Rats with Heart Failure after Myocardial Infarction
ZHANG Shandong
RUAN Zixuan
CHENG Xinyi
QIAN Yi
ZHANG Yexiang
Abstract：Objective:To investigate the effect of Fuling Wenshen Capsule on heart failure after myocardial infarction based on the phosphatidylinositol-3-kinase(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(mTOR)autophagy signaling pathway.Methods:The rat model of heart failure was replicated by intraperitoneal injection of isoproterenol.The rats were divided into model group,traditional Chinese medicine group(Fuling Wenshen Capsule),western medicine group(enalapril tablet),and control group using the random number table method.Serum troponin T(cTnT)and serum N-terminal brain natriuretic peptide(NT-proBNP)were measured by enzyme linked immunosorbent assay(ELISA)after 14 days of continuous administration.The morphological changes of cardiomyocytes were observed using hematoxylin-eosin(HE)and Masson's staining.The phosphorylation levels of autophagy marker proteins,PI3K,AKT,and mTOR were detected by Western blot.Results:The values of cTnT and NT-proBNP significantly increased in rats with heart failure induced by isoproterenol after MI.The fibrosis of myocardial tissue increased,the structure of myocardial tissue disordered,autophagy obviously activated,and the number of autophagosomes increased.After the intervention with Fuling Wenshen Capsule,the levels of cTnT and NT-proBNP were significantly decreased(P＜0.05),cardiac function was improved,the degree of myocardial fibrosis was reduced,the number of autophagosomes was decreased,and the levels of PI3K,AKT,mTOR,and their phosphorylation levels were significantly increased(P＜0.05).Conclusion:Fuling Wenshen Capsule could improve ventricular remodeling and cardiac function in rats with heart failure after MI,and its mechanism related to regulating the PI3K/AKT/mTOR signaling pathway and inhibiting autophagy.
Keywords：myocardial infarctionheart failureFuling Wenshen Capsuleventricular remodelingphosphatidylinositol-3-kinaseprotein kinase Bmammalian target of rapamycin
Publication Date：2025-08-10
Online Publishing Date：2025-08-20(First online date of this platform, not the publication date of the document)
Pages：8( 2283-2290 )