The Effect of Mingjun Powder on the TLR4/MyD88/NF-κB Signaling Pathway in Atherosclerotic Rats
CHE Sihua
YANG Na
ZHENG Wenyu
ZHANG Shumao
SHI Guijun
Abstract：Objective:To explore the effect of Mingjun Powder on atherosclerotic rats based on the Toll-like receptor 4(TLR4)/myeloid differentiation protein 88(MyD88)/nuclear factor-κB(NF-κB)signaling pathway.Methods:The atherosclerotic model of rats was established by high-fat feed combined with vitamin D3 method.The rats were divided into the blank group,the model group,the Western medicine group[atorvastatin 1 mg/(kg·d)],the low-dose Mingjun Powder group[2.35 mg/(kg·d)],the medium-dose Mingjun Powder group[4.70 mg/(kg·d)],and the high-dose Mingjun Powder group[9.40 mg/(kg·d)],with 10 rats in each group.After gavaging for eight weeks,the levels of serum total cholesterol(TC),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),and low-density lipoprotein cholesterol(LDL-C)were detected.Hematoxylin-eosin(HE)staining was used to observe the histological morphological changes of the thoracic aorta in rats.The expressions of TLR4,MyD88 and NF-κB mRNA in the aorta of rats were detected by reverse transcription polymerase chain reaction(RT-PCR).The expressions of TLR4,MyD88,and NF-κB proteins in the aorta of rats were detected by Western Blot.Results:Compared with the model group,the levels of serum TC,TG,and LDL-C in the Western medicine group,the medium-dose Mingjun Powder group,and the high-dose Mingjun Powder group decreased,and the level of HDL-C increased(P＜0.05 or P＜0.01).The expressions of TLR4,MyD88,NF-κB mRNA and protein in the aortic tissues of rats in the Western medicine group and each dose Mingjun Powder groups all decreased(P＜0.05 or P＜0.01).Conclusion:Mingjun Powder might play an anti-atherosclerotic effect by inhibiting the TLR4/MyD88/NF-κB signaling pathway.
Keywords：atherosclerosisMingjun PowderToll-like receptor 4/myeloid differentiation protein 88/nuclear factor-κB signaling pathwayblood lipidratsexperimental study
Publication Date：2025-07-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 2133-2138 )