Effects of Panax Notoginseng Saponins on Myocardial Ischemia-reperfusion Injury in Rats and the Mechanism of Anti-pyroptosis
ZHAO Weikun
XU Tongtong
LYU Xiangwei
QIN Yang
LU Fengxia
LI Yuting
HUANG Yuli
MO Qi
XU Huaxin
LYU Fei
Abstract：Objective:To observe the effect of Panax notoginseng saponins(PNS)on myocardial ischemia-reperfusion(IR)injury and explore its mechanism related to the regulation of cardiomyocyte pyroptosis.Methods:Eighteen adult male SD rats were divided into sham group,IR group and PNS group by random number table method,with 6 rats in each group.The model was prepared by ligating the left anterior descending coronary artery(LAD)of rats,with ischemia for 30 minutes and reperfusion for 2 hours.The rats in the sham group and the IR group were intraperitoneally injected with normal saline(10 mL/kg),while those in the PNS group were intraperitoneally injected with PNS(50 mg/kg)30 minutes before modeling.The degree of myocardial injury was observed by hematoxylin-eosin(HE)staining.The expression of cysteine aspartate specific protease-1(Caspase-1)was detected by immunohistochemistry.The expressions of Caspase-1 and gasdermin D(GSDMD)protein contents in myocardial tissue were detected by Western Blot.Results:Compared with the sham group,the degree of myocardial injury in the IR group was aggravated,and the protein expressions of Caspase-1 and GSDMD increased(P＜0.05).Compared with the IR group,the degree of myocardial injury in the PNS group was alleviated,and the protein expressions of Caspase-1 and GSDMD decreased(P＜0.05).Conclusion:Panax notoginseng saponins could alleviate myocardial IR injury in rats,and its mechanism might relate to the inhibition of cardiomyocyte pyroptosis.
Keywords：myocardial ischemia reperfusion injurypanax notoginseng saponinspyroptosiscysteine aspastic acid-specific protease-1(Caspase-1)gasdermin D(GSDMD)ratsexperimental study
Publication Date：2025-07-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 2128-2132 )