Mechanism of Hawthorn Extract Regulating T-cell Immunity and Lipid Metabolism in Hyperlipidemic Mice
FU Yu
YANG Ying
Abstract：Objective:To explore the mechanism of hawthorn extract regulating T-cell immunity and lipid metabolism in hyperlipidemic mice.Methods:Sixty healthy male 6-week-old specific pathogenic(SPF)grade C57BL/6J mice were adaptively raised for one week and then randomly divided into the normal control group,high-fat diet(HFD)group,HFD+high-dose hawthorn extract group,HFD+medium-dose hawthorn extract group,HFD+low-dose hawthorn extract group,and HFD+atorvastatin group.The normal control group was given ordinary feed and raised normally for 8 weeks,while the mice in the HFD group were fed with high-fat feed powered by 60％fat for 8 weeks.Mice in the HFD+high-dose hawthorn extract group,HFD+medium-dose hawthorn extract group,and HFD+low-dose hawthorn extract group were gavaged with 5.2,2.6,and 1.3 g/kg hawthorn extract,respectively after 4 weeks of high-fat rearing,once a day.Mice in the atorvastatin group were given oral administration of atorvastatin at a dose of 10 mg/kg by gavage after 4 weeks of high-fat feeding,once a day.The control group and the HFD group were gavaged with the same volume of normal saline at the same volume.The intervention period was 4 weeks.The mRNA and protein expression levels of CD36 and peroxidase proliferation-activated receptor γ(PPARγ)were detected by Western Blot and reverse transcription polymerase chain reaction(qRT-PCR).Flow cytometry was used to detect the proportions of regulatory T cells(Treg),inhibitory helper T cells(Th17),and follicular helper T cells(Tfh)in the spleen tissue of mice.Results:Compared with the normal group,the body weight of mice in the HFD group increased(P＜0.01);the expression levels of CD36,PPARγ mRNA,protein increased(P＜0.01);the proportion of Th17 cells in the spleen increased,and the percentages of Treg and Tfh cells increased(P＜0.01).Compared with the mice in the HFD group,the expression levels of CD36,PPARγ mRNA,and protein in the model group mice intervened with hawthorn extract and atorvastatin decreased(P＜0.01),the percentages of Treg and Tfh cells increased(P＜0.01),and the percentage of Th17 cells decreased(P＜0.01).The effect of the HFD+high-dose hawthorn extract group and the atorvastatin group were significant(P＜0.05 or P＜0.01).Conclusion:Hawthorn extract can improve the imbalance of Treg,Th17 and Tfh cells and the expression of CD36,PPARγ receptors in mice with dyslipidemia models.Lipid regulation by hawthorn extract may be related to T-cell immunity and lipid metabolism differentiation.
Keywords：dyslipidemiaT cell immunityhawthorn extractperoxisome proliferator-activated receptor γexperimental study
Publication Date：2025-07-10
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 1961-1970 )