Effect of Yanggan Yishui Granules on the Apoptosis of HK-2 Cells in the Angiotensin Ⅱ-Induced Hypertensive Renal Damage Model
XIA Ran
LIU Pan
KONG Jingjing
YUAN Liang
DAI Xiaohua
WANG Jing
YANG Fan
Abstract：Objective:To explore the effect and mechanism of Yanggan Yishui Granules on angiotensin Ⅱ(Ang Ⅱ)-induced apoptosis of HK-2 cells.Methods:The HK-2 hypertensive renal damage(HRD)cell model was induced by AngⅡ and divided into the normal group,the AngⅡ group and the YGYS group.The key target gene interleukin-6(IL-6)was silenced or overexpressed.CCK-8 method was used to detect the optimal serum concentration of drug for model establishment and therapeutic effect.The mRNA expression levels of IL-6,tumor necrosis factor-α(TNF-α),serum protein(ALB),epidermal growth factor receptor(EGFR),angiotensin-converting enzyme(ACE),and matrix metal peptidase 2(MMP2)were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction(RT-qPCR).The expression levels of hypoxia-inducible factor-1 α(HIF-1α)and cysteine proteolytic ase-9(Caspase-9)were detected by Western Blot and RT-qPCR.The apoptosis rate of cells was detected by flow cytometry.Results:Yanggan Yishui Granules could reduce the levels of inflammatory factors such as TNF-α,ALB,EGFR,ACE,and MMP2 in the HK-2 cell model induced by AngⅡ(P＜0.05),with the most significant reduction in the level of IL-6(P＜0.01).Silencing IL-6,the expression levels of HIF-1α and Caspase-9 proteins and mRNA decreased(P＜0.01),and the apoptosis rate decreased(P＜0.01).Based on the AngⅡ group,overexpression of IL-6 could antagonize the effect of Yanggan Yishui Granules in down-regulating the expression levels of HIF-1α,Caspase-9 protein and mmRNA(P＜0.01)and reducing cell apoptosis(P＜0.05).Conclusion:Yanggan Yishui Granules can down-regulate the apoptosis of HK-2 cells induced by AngⅡ,and the underlying mechanism may be related to the inhibition of the IL-6/HIF-1α/Caspase-9 signaling axis.
Keywords：hypertensive renal damageYanggan Yishui Granulesinflammatory responseinterleukin-6hypoxia-inducible factor-1αcysteine proteolytic ase-9experimental study
Publication Date：2025-06-10
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：11( 1641-1651 )