The Effert of Exocarpium Citri Grandis Mediated NF-κB Signaling Pathway on Brain Tissue Injury and Cognitive Function in Mice with Cerebral Ischemia-reperfusion Injury
LIU Xueshuang
LUO Jun
WANG Kai
LI Wei
WANG Pu
Abstract：Objective:To explore the effects of exocarpium citri grandis(ECG)on brain tissue injury and cognitive function in mice with cerebral ischemia-reperfusion injury(CIRI).Methods:SD mice were randomly divided into sham group,model group,ECG low-dose group,ECG mid-dose group,ECG high-dose group,and nimodipine group,with 10 mice in each group.The model of middle cerebral artery occlusion was established in all groups except sham group.The moisture content of brain was measured by dry/wet method.Longa method was used to evaluate nerve function injury in mice.Hematoxylin-eosin(HE)staining was used to detect the pathological injury of brain tissue.Platform test detected the number of mistakes.Y Maze test recorded the entry times of new arms.The levels of interleukin-6(IL-6),inducible nitric oxide synthase(iNOS),and tumor necrosis factor(TNF)-α were detected by enzyme-linked immunosorbent assay(ELISA).The protein expression levels of brain-derived neurotrophic factor(BDNF),nerve growth factor(NGF),Toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88),nuclear transcription factor-κB(NF-κB)p65,and phosphorylated nuclear transcription factor-κB(p-NF-κB)p65 were examined using Western Blot.Results:Compared with sham group,brain water content,neurological deficit score,times of mistakes,MDA content,IL-6,iNOS,and TNF-α levels increased in model group,TLR4,MyD88 protein expression levels and NF-κB p65/p-NF-κB p65 ratio increased,number of new arm entry,the expression levels of BDNF and NGF protein and SOD content decreased(P＜0.05).Compared with model group,brain water content,neurological deficit score,times of mistakes,MDA content and IL-6,iNOS,and TNF-αlevels of mice in the medium dose group,high dose group,and nimodipine group decreased,the expression levels of TLR4 and MyD88 protein and the ratio of NF-κB p65/P-NF-κB p65 decreased,while number of new arm entry,the expression levels of BDNF and NGF protein,and SOD content increased(P＜0.05).Conclusion:ECG may mediate NF-κB signaling pathway to protect brain tissue and improve cognitive function in mice with CIRI.
Keywords：cerebral ischemia-reperfusion injuryexocarpium citri grandisinflammatory factornuclear transcription factor-κB signaling pathway
Publication Date：2025-05-10
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1331-1337 )