Protective Effect of Lycopene Upregulation of miR-141-3p on Hypoxia/Reoxygenation Injury of Human Cardiomyocytes
LI Mengyi
HE Wenjing
CAI Sisi
XIAO Wenjing
Abstract：Objective:To investigate the protective effect of lycopene on human cardiomyocytes AC16 against hypoxia/reoxygenation(H/R)injury by upregating miR-141-3p.Methods:After H/R treatment,the third generation of logarithmic AC16 cells were divided into injury group,5,10,20 μg/mL lycopene group,and other cells without H/R treatment were selected as normal group.Cell survival rate was detected by CCK-8 assay.Cell apoptosis rate was detected by flow cytometry.Lactate dehydrogenase(LDH)and creatine kinase isoenzyme(CK-MB)levels were detected by enzyme-linked immunosorbent assay(ELISA).Real-time fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to detect the expression of miR-141-3p,and Western Blot was used to detect the expression of B-cell lymphoma-2(Bcl-2)and Bcl-2 associated X protein(Bax).After H/R treatment,the third generation of logarithmic AC16 cells were divided into miR-141-3p mimic NC group,miR-141-3p mimic group,lycopene+miR-141-3p inhibitor NC(20 μg/mL lycopene treatment after transfection with inhibitor NC 24 h)and lycopene+miR-141-3p inhibitor group(20 μg/mL lycopene treatment after transfection with inhibitor 24 h).The above methods were used to detect the expression level of miR-141-3p,cell survival rate and apoptosis rate,LDH and CK-MB levels,and the expression levels of Bax and Bcl-2 proteins.Results:Compared with normal group,cell survival rate,Bcl-2 protein expression and miR-141-3p expression decreased in injured group,while apoptosis rate,Bax protein expression and CK-MB and LDH levels increased(P＜0.05).Compared with injury group,cell survival rate,Bcl-2 protein expression and miR-141-3p expression in 5,10 and 20 μg/mL lycopene groups increased,while apoptosis rate,Bax protein expression and CK-MB and LDH levels decreased(P＜0.05).Compared with miR-141-3p mimic NC group,cell survival rate,Bcl-2 protein expression and miR-141-3p expression increased in miR-141-3p mimic group,while apoptosis rate,Bax protein expression and CK-MB and LDH levels decreased(P＜0.05).Compared with lycopene+miR-141-3p inhibitor NC group,cell survival rate,Bcl-2 protein expression and miR-141-3p expression decreased in lycopene+miR-141-3p inhibitor NC group,while apoptosis rate,the expression of Bax protein and the levels of CK-MB and LDH increased(P＜0.05).Conclusion:Lycopene can inhibit H/R-induced cardiomyocyte injury through up-regulation of miR-141-3p expression.
Keywords：hypoxia/reoxygenationcardiomyocytesmiR-141-3plycopene
Publication Date：2025-05-10
Pages：7( 1318-1324 )