Effects of Exogenous NO Donor on Apoptosis,Troponin,and Histone Deacetylase of H9c2 Cells Induced by Hypoxia/Reoxygenation
LI Li
WANG Zhixin
LYU Yaxuan
GUO Yanqing
Abstract：Objective:To explor the effect of exogenous nitric oxide(NO)donor on hypoxia/reoxygenation induced H9c2 cell damage.Methods:H9c2 cells were divided into control group,hypoxia/reoxygenation group,and hypoxia/reoxygenation+NO group[H9c2 cells treated with exogenous NO donor sodium nitroprusside(SNP)].Cell viability was detected by cell counting reagent(CCK-8).The apoptosis rate was detected by flow cytometry.The expression of cardiac troponin T(cTnT)was detected by immunofluorescence.The expression of troponin C(TNNC1)in slow muscle,troponin I(TNNI2)in fast muscle,and troponin T(TNNT2)in skeletal muscle were detected by Western Blot.The level of histone deacetylases(HDACs)was detected by enzyme-linked immunosorbent assay(ELISA)kit.Results:H9c2 cells were treated with 1 mmol/L SNP solution for 48 h,and the cell activity was the highest.Compared with the control group,the apoptosis rate of hypoxia/reoxygenation group increased(P＜0.01),while the apoptosis rate of hypoxia/reoxygenation+NO group decreased(P＜0.05),and the apoptosis rate of hypoxia/reoxygenation+NO group lower than that of the hypoxia/reoxygenation group(P＜0.01).Compared with the control group,the expression levels of TNNC1,TNNI2,and TNNT2 protein in hypoxia/reoxygenation group increased(P＜0.05),the expression levels of TNNI2 and TNNT2 protein decreased compared with those of the hypoxia/reoxygenation group(P＜0.01).The HDACs concentration in hypoxia/reoxygenation group was lower than that in the control group,and the HDACs concentration in hypoxia/reoxygenation+NO group was higher than that in the hypoxia/reoxygenation group,the differences were statistically significant(P＜0.01).Conclusion:Exogenous NO donors may protect H9c2 cardiomyocytes from hypoxia/reoxygenation injury by inhibiting myocardial apoptosis,reducing troponin expression,and upregating HDACs.
Keywords：exogenous nitric oxide donorhypoxia/reoxygenationH9c2 cellsischemia reperfusion injurysodium nitroprussideexperimental study
Publication Date：2025-04-10
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1022-1027 )