Effect of Sulforaphane Regulating PI3K/AKT/VEGF Signaling Pathway on Vascular Endothelial Function in Pregnancy-induced Hypertension Rats
LIU Zheng
LI Jing
Abstract：Objective:To explore the effect of sulforaphane(SFN)on vascular endothelial function in pregnancy-induced hypertension(PIH)rats.Methods:Thirty pregnant SD rats free of specific pathogen(SPF)were randomly divided into control group,PIH group,SFN low-dose group(15 mg/kg),SFN high-dose group(30 mg/kg),and LY294002 group(30 mg/kg SFN+5 mg/kg LY294002).PIH rat model was established and systolic blood pressure was measured.Bicinchoninic acid(BCA)method was used to determine the urine protein content.Enzyme-linked immunosorbent assay(ELISA)was used to measure serum tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),malondialdehyde(MDA),superoxide dismutase(SOD),glutathione(GSH),vascular endothelial growth factor(VEGF),vascular cell adhesion molecule 1(VCAM-1),endothelin-1(ET-1),and nitric oxide(NO).Western Blot was used to detect protein expression levels of phosphoinositide 3-kinase(PI3K),phosphorylated PI3K(p-PI3K),protein kinase B(AKT),phosphorylated AKT(p-AKT),and vascular endothelial growth factor(VEGF)in placenta tissue.Results:Compared with the control group,the systolic pressure,urine protein content,levels of TNF-α,IL-1β,IL-6,MDA,ET-1,and VCAM-1 of the rats in the PIH group increased(P＜0.05),the protein levels of VEGF,NO,SOD,GSH,p-PI3K/PI3K,p-AKT/AKT,and VEGF decreased(P＜0.05).Compared with the PIH group,the systolic pressure,urine protein content,levels of TNF-α,IL-1β,IL-6,MDA,ET-1,and VCAM-1 of rats in the SFN low and high dose groups decreased(P＜0.05),the protein levels of VEGF,NO,SOD,GSH,p-PI3K/PI3K,p-AKT/AKT,and VEGF increased(P＜0.05).The PI3K inhibitor LY294002 attenuated the protective effect of SFN on vascular endothelial function in PIH rats.Conclusion:SFN shows a protective effect on vascular endothelial function in PIH rats,which may be related to the activation of PI3K/AKT/VEGF signaling pathway.
Keywords：pregnancy-induced hypertensionsulforaphanevascular endothelial functionexperimental research
Publication Date：2025-04-10
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1009-1014 )