The Improvement of Myocardial Energy Metabolism by Regulating Mitochondrial Function of Shexiang Baoxin Pills Based on AMPK/PGC-1α Pathway
SONG Xiaolong
PAN Renyou
GU Yuexing
SONG Jun
TU Dongyun
WU Deming
NI Qimeng
Abstract：Objective:To observe the effect of Shexiang Baoxin pills on myocardial energy metabolism of coronary heart disease.Methods:The H9c2 cardiomyocytes were divided into Control group,Model group(H9c2 cardiomyocytes treated with hypoxia/reoxygenation),low-dose Shexiang Baoxin pills group(SBP-L group,100 μg/L Shexiang Baoxin pills treatment),medium-dose Shexiang Baoxin pills group(SBP-M group,200 μg/L Shexiang Baoxin pills treatment),high-dose Shexiang Baoxin pills group(SBP-H group,400 μg/L Shexiang Baoxin pills treatment)and trimetazidine group(TMZ group,trimetazidine treatment).The cell viability was detected Using cell count kit-8(CCK-8).The contents of mitochondrial adenosine triphosphate(ATP)was detected by adenosine 5′-triphosphate bioluminescence assay kit.Mitochondrial membrane potential was detected with 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazole carbocyanine iodide(JC-1)staining kit.Reactive oxygen species were detected by 2′,7′-dichlorodihydrofluorescein diacetate(DCFH-DA)staining.The contents of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were detected by enzyme-linked immunosorbent assay(ELISA).Apoptosis detection kit was used to evaluate apoptosis.The expressions of apoptosis-related genes Bcl-2 associated X protein(Bax),B lymphoblastoma-2(Bcl-2),Caspase-3 and Caspase-9 were detected by Western Blot.The expression of peroxisome proliferator activating receptor γ coactivator 1α(PGC-1α)and adenosine diphosphate(AMP)-activated protein kinase(AMPK)were detected by Western Blot and real-time quantitative fluorescent polymerase chain reaction(qRT-PCR).The SBP-H group was treated with AMPK inhibitor(CC)to detect cell apoptosis,mitochondrial ATP content,PGC-1α and AMPK expression.Results:The cell activity,mitochondrial ATP content,mitochondrial membrane potential,SOD content,GSH-Px content,PGC-1α and phosphorylated AMPK(p-AMPK)expression in the model group were significantly decreased,while ROS,MDA and cell apoptosis were significantly increased.The above cell function and protein expression were reversed in the SBP-L group,SBP-M group,SBP-H group and TMZ group,and the effects of SBP-H group and TMZ group were significant.After addition of AMPK inhibitor in the SBP-H group,apoptosis inhibition,PGC-1α and up-regulation of p-AMPK expression were reversed.Conclusion:Shexiang Baoxin pills could promote mitochondrial function through AMPK/PGC-1α pathway,and then improve energy metabolism of cardiomyocytes.
Keywords：coronary heart diseaseShexiang Baoxin pillsH9c2 cardiomyocytesadenosine diphosphate(AMP)-activated protein kinaseperoxisome proliferator activating receptor γ coactivator 1αmitochondriaenergy metabolismexperimental study
Publication Date：2025-03-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 848-856 )