The Mechanisms of Astragalus Polysaccharide for Protecting Cardiomyocytes against H2O2-induced Apoptosis
WU Shengjie
WAN Siyuan
WANG Zhewen
YANG Shiyu
WANG Yihui
GUO Haidong
LI Han
Abstract：Objective:To investigate the protective effect of Astragalus polysaccharide on rat cardiomyocytes(H9c2)against hydrogen peroxide(H2O2)-induced apoptosis.Methods:H9c2 cells were cultured in vitro.After pretreatment with Astragalus polysaccharide,the oxidative stress model was simulated by H2O2.The effects of Astragalus polysaccharides on the proliferation of H9c2 cells and their resistance to oxidative stress induced by H2O2 were detected by cell counting kit(CCK-8)and terminal deoxynucleotide transferase mediated dUTP notch end labeling(TUNEL).The levels of phosphorylated protein kinase B(p-AKT)and phosphorylated extracellular regulatory protein kinase 1/2(p-ERK1/2)were detected by Western Blot to explore the protective effect of Astragalus polysaccharide on H9c2 cells in vitro.Results:The 100 μg/mL Astragalus polysaccharide could significantly promote the proliferation activity of H9c2 cells.The apoptosis rate of H9c2 cells was about 50%due to 350 μmol/L H2O2.In vitro oxidative stress model was established using 350 μmol/L H2O2.CCK-8 activity detection showed that 100 μg/mL of Astragalus polysaccharide showed a significant protective effect on H9c2 cells against oxidative stress damage induced by H2O2.The results of Western Blot analysis showed that compared with Control group and H2O2 group,Astragalus polysaccharide+H2O2 group p-AKT and p-ERK1/2 protein expression activated.Phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)and mitogen-activated protein kinase(MAPK)/extracellular regulatory protein kinase(ERK)signaling pathway inhibitors LY294002 and U0126 inhibited the activation of the signaling pathway by Astragalus polysaccharides.After the treatment of H9c2 cells with Astragalus polysaccharide and H2O2,TUNEL detection showed that the number of apoptosis of H9c2 cells in the Astragalus polysaccharide+H2O2 group decreased(P＜0.05).Conclusion:Astragalus polysaccharide could protect H9c2 cells against oxidative stress induced by H2O2,and its mechanism might be related to the activation of PI3K/AKT and MAPK/ERK signaling pathways.
Keywords：H9c2 cellsAstragalus polysaccharideoxidative stresscell apoptosisphosphatidylinositol 3-kinase/protein kinase B signaling pathwaymitogen-activated protein kinase/extracellular regulatory protein kinase signaling pathwayexperimental study
Publication Date：2025-03-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 841-847 )