Effect of miR-30c/Runx2 on Vascular Calcification in Coronary Heart Disease Based on lncRNA MALAT1
LI Qian
WU Dan
HU Can
LI Hongguo
XIAO Xiaoqiao
WEN Qing
ZHOU Ping
Abstract：Objective:To investigate the effect of long non-coding RNA(lncRNA)transmission-associated lung adenocarcinoma transcript 1(MALAT1)on coronary artery disease(CHD)vascular calcification through miR-30c/Runx2 pathway.Methods:Human vascular smooth muscle cells(VSMCs)were induced to estabolish the vascular calcification model in vitro.Dwarf associated transcription factor 2(Runx2)and α-smooth muscle actin(α-SMA)were detected by alizarin red S staining,calcium content determination and protein study.The effect of MALAT1 knockdown and MALAT1,miR-30c and Runx2 up-regulation on VSMCs calcification was determined.Dual luciferase reporting was used to confirm the relationship between MALAT1 and miR-30c or miR-30c and Runx2.Quantitative reverse transcription polymerase chain reaction(qRT-PCR)and Western Blot were used to detect gene and protein expression.Results:In calcified VSMCs,MALAT1 increased,miR-30c decreased,and MALAT1 knock-down inhibited VSMCs calcification.Up-regulation of MALAT1 promoted calcification of VSMCs.The effect of MALAT1 overexpression on calcification of VSMCs was reversed by up-regulation of miR-30c,while up-regulation of Runx2 again reversed.Dual luciferase assay confirmed the direct interaction between MALAT1 and miR-30c,and Runx2 was the direct target of miR-30c.Conclusion:MALAT1 overexpression promotes VSMC calcification,in part by modulating the miR-30c/Runx2 axis.
Keywords：coronary heart diseasevascular calcificationtransmission-associated lung adenocarcinoma transcript 1dwarf associated transcription factor 2
Publication Date：2025-03-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 690-696 )