Astragaloside Improves Hemorheology of Limb Arteriosclerosis Obliterans in Rats by Regulating Nrf2/HO-1 Pathway
SUN Yunchao
GUO Na
WEI Jin
PANG Nan
WANG Yuqing
SHI Lin
TIAN Yueting
Abstract：Objective:To explore the therapeutic effect of astragaloside on arteriosclerosis obliterans(ASO)in rats.Methods:ASO model was established by high-fat diet and intima injury of cryptoartery.The rats successfully modeled were randomly divided into model group,astragaloside low-dose group,and astragaloside high-dose group,with 10 rats in each group.In addition,a sham operation group(10 rats)was set up.Whole blood viscosity,red blood cell aggregation index,and red blood cell rigidity index were analyzed by haemorheological analyzer.Blood levels of total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C),and low-density lipoprotein cholesterol(LDL-C)were measured using a fully automated biochemical analyzer.Hematoxylin-eosin(HE)and elastic-van gieson(EVG)staining were used to detect the pathological morphological changes of arterial vessels in rats.Serum interleukin-6(IL-6),interleukin-1 β(IL-1 β),and tumor necrosis factor-α(TNF-α)were detected by enzyme-linked immunosorbent assay(ELISA).The expressions of nuclear factor E2-associated factor 2(Nrf2),Kelch-like ECH associated protein 1(Keap1)and heme oxygenase-1(HO-1)were detected by Western Blot.Results:Although the astragaloside group showed endothelial structure damage and lumen stenosis,compared with the model group,it reduced.Compared with the model group,the blood viscosity of astragaloside group decreased and the blood fluidity increased.Compared with model group,the levels of TG,TC,LDL-C,TNF-α,IL-1β,and IL-6 in astragaloside group decreased,while HDL-C increased(P＜0.05).Compared with model group,the protein levels of Nrf2,Keap1,and HO-1 in astragaloside group increased(P＜0.05).Conclusion:Astragaloside can regulate Nrf2/HO-1 pathway,inhibit inflammation,improve lipid metabolism and hemorheology indexes,and alleviate arterial tissue damage in ASO rats.
Keywords：arteriosclerosis obliteransastragalosidelipid metabolismhemorheology
Publication Date：2025-03-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 678-683 )