circPRMT5 Targets miR-376a-3p to Regulate Proliferation and Apoptosis of Glioma U251 Cells
HUANG Chao
FANG Xinggang
CHEN Lu
Abstract：Objective:To explore the effect and molecular mechanism of circPRMT5 on the proliferation and apoptosis of glioma U251 cells.Methods:A total of 37 specimens of brain glioma tissues and 37 specimens of brain tissues from patients with craniocerebral trauma were selected from May 2020 to May 2022.U251 glioma cells were divided into si-NC group,si-circPRMT5 group,pcDNA group,pcDNA-circPRMT5 group,miR-NC group,miR-376a-3p group,si-circPRMT5+anti-miR-NC group,and si-circPRMT5+anti-miR-376a-3p group.Real-time fluorescence quantitative PCR(RT-qPCR)was used to detect the expressions of circPRMT5 and miR-376a-3p.Methyl thiazolyl tetrazolium(MTT)was used to detect cell activity.Flow cytometry was used to detect apoptosis.The expression of proteins related to cell proliferation and apoptosis was detected by Western Blot.The dual luciferase reporter assay was used to detect the relationship between circPRMT5 and miR-376a-3p.Results:Compared with the brain tissue of patients with traumatic brain injury,the expression level of circPRMT5 in glioma tissue was increased(2.55±0.18 vs 1.00±0.07,P＜0.05),and the expression level of miR-376a-3p was decreased(0.42±0.05 vs 1.00±0.06,P＜0.05).After inhibiting the expression of circPRMT5,cell viability(0.32±0.03 vs 0.74±0.06)was decreased,cell apoptosis rate[(22.37±1.83)%vs(7.47±0.62)%]was increased,the expression levels of CyclinD1 and B-cell lymphoma/leukemia-2(Bcl-2)were decreased,and the expression levels of cyclin-dependent kinase inhibitor 1A(p21)and Bcl-2 related X(Bax)protein were increased(P＜0.05).After overexpression of miR-376a-3p,cell viability(0.41±0.04 vs 0.76±0.07)was decreased,and cell apoptosis rate[(19.51±1.40)%vs(7.37±0.53)%]was increased,the expression levels of CyclinD1 and Bcl-2 were decreased,and the expression levels of p21 and Bax were increased(P＜0.05).Compared with the miR-NC group,the luciferase activity of cells transfected with WT-circPRMT5 in the miR-376a-3p group was decreased(0.59±0.04 vs 1.03±0.07,P＜0.05),while the luciferase activity of cells transfected with MUT-circPRMT5 was no significant change(1.00±0.05 vs 1.02±0.08,P＞0.05).Conclusion:Inhibition of circPRMT5 expression may inhibit the proliferation of glioma U251 cells and promote apoptosis by targeting up-regulation of miR-376a-3p.
Keywords：gliomaproliferationapoptosiscircPRMT5miR-376a-3pexperimental research
Publication Date：2025-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 547-554 )