Effects of Qishen Yiqi Droplet on Coronary Blood Flow Perfusion and Cardiac Function in Diabetes Rats with Myocardial Ischemia
LIU Tiantian
LIU Xin
MA Lina
GU Yuanyuan
ZHANG Yanli
GONG Ying
Abstract：Objective:To explore the effect of Qishen Yiqi Droplet on coronary blood flow and cardiac function in diabetes rats with myocardial ischemia.Methods:Wistar rats were randomly divided into control group,diabetes myocardial ischemia group,positive drug group[Nicorandil Tablets 3.15 mg/(kg·d)],and Qishen Yiqi Droplet group[157.5 mg/(kg·d)].The control group was intraperitoneally injected with sodium citrate buffer,and the other three groups were intraperitoneally injected with streptozotocin[50 mg/(kg·d)]every day for three consecutive days to establish the diabetes model.The myocardial ischemia model was established by oral administration of Nicorandil Tablets and Qishen Yiqi Droplet for 17 days.At the 16 th and 17 th days,isoproterenol hydrochloride 50 mg/(kg·d)was subcutaneously injected on the nape of the neck of rats,and the control group was subcutaneously injected with equivalent volume of normal saline.Cardiac ultrasound was used to detect the cardiac function of rats in each group,and ventricular septal coronary blood flow was recorded by pulsed wave Doppler mode.The serum levels of cardiac troponin I(cTnI),creatine kinase isoenzyme(CK-MB),glutamic-oxaloacetic transaminase(GOT),and nitric oxide(NO)were measured.Cardiac pathological changes,myocardial apoptosis,glycogen accumulation,α-smooth muscle actin(α-SMA),and von Willebrand factor(vWF)expression were observed in each group.Western Blot was used to detect the protein expressions of silent information regulator 1(Sirt1),protein kinase B(AKT),AMP-activated protein kinase α(AMPKα),peroxidase body increasing biological activated receptor-gammaa auxiliary activation factor-1α(PGC-1α),and endothelial nitric oxide synthase(eNOS)in cardiac tissues.Results:Compared with the control group,the ejection fraction(EF),fraction shortening(FS),and coronary blood flow of diabetes rats with myocardial ischemia were significantly reduced,and the levels of CK-MB,cTnI,and GOT in serum were significantly increased.There was a significant loss of muscle fibers in myocardial cells,an increase in inflammatory cell infiltration,glycogen deposition,and an increase in the number of myocardial apoptosis,The number of new blood vessels labeled with α-SMA and vWF reduced.The expressions of Sirt1,p-AKT/AKT,p-AMPKα/AMPKα,PGC-1α,pS1177-eNOS were elevated,while the levels of eNOS and NO decreased.Qishen Yiqi Droplet can significantly improved myocardial ischemia and hypoxia in diabetes and cardiac function damage.Conclusion:Qishen Yiqi Droplet may activate Sirt/AMPK/eNOS signal,promote NO release,inhibit cardiomyocyte apoptosis,reverse vascular endothelial injury,increase endothelial cell migration and invasion function,promote angiogenesis in ischemic area,thereby increasing coronary blood flow perfusion and restoring cardiac function in diabetes rats with myocardial ischemia.
Keywords：myocardial ischemiadiabetesQishen Yiqi Dropletsilent information regulator 1Sirt1AMP-activated protein kinaseAMPKendothelial nitric oxide synthaseeNOSexperimental study
Publication Date：2025-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 539-546 )