Molecular Mechanism Study of Shexiang Tongxin Dripping Pills in Improving Myocardial Ischemia-reperfusion Injury:a Network Pharmacology Analysis and Animal Experiment Validation
NIU Chaofeng
ZHANG Lijing
LIU Birong
XIAO Di
LIU Yong
ZHANG Chao
ZHANG Peiyu
LI Meng
Abstract：Objective:To explore the molecular mechanisms underlying the therapeutic effects of Shenxiang Tongxin Dripping Pills on myocardial ischemia-reperfusion injury(MIRI)using a combination of network pharmacology analysis and animal experiments.Methods:The active ingredients of Shexiang Tongxin Dripping Pills were retrieved from the traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP)and BATMAN TCM databases,and their corresponding target proteins were screened.Additionally,MIRI-related target proteins were collected,and the intersection of these two sets of targets was obtained.Protein-protein interaction(PPI)networks were constructed,and gene ontology(GO)and Kyoto Encyclopaedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed.Furthermore,compound-target and herb-target-pathway networks were visualized.Animal experiments were conducted to validate the potential mechanisms.Results:The results showed that Shexiang Tongxin Dripping Pills contained 122 chemical components and 485 related target proteins,of which 165 were associated with MIRI.PPI network analysis revealed key targets such as protein kinase B1(AKT1),tumor necrosis factor(TNF),TP53,signal transducer and activator of transcription 3(STAT3),JUN,interleukin(IL)6,and IL1B.KEGG pathway enrichment analysis indicated there were imultiple signaling pathways in the therapeutic effects of Shexiang Tongxin Dripping Pills.The results of the animal experiments were consistent with the predictions of the network pharmacology analysis.The results of genome sequencing and protein detection showed that Shexiang Tongxin Dropping Pills may treat MIRI by regulating hypoxia-inducible factor(HIF)-1α and vascular endothelial growth factor(VEGF).Conclusion:This study comprehensively elucidated the effective components,molecular mechanisms,and potential targets of Shexiang Tongxin Dripping Pills for the treatment of MIRI.
Keywords：myocardial ischemia-reperfusion injuryShexiang Tongxin Dropping Pillsnetwork pharmacologymechanism
Publication Date：2025-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：12( 513-524 )