The Mechanism of Shexiang Baoxin Pill in the Intervention of Myocardial Fibrosis in Rats with Myocardial Infarction Induced by Isoproterenol
LAI Qi
YANG Ting
CHU Chun
YANG Jun
Abstract：Objective:To observe the effect of Shexiang Baoxin Pill(SBP)on improving myocardial fibrosis in rats with isoproterenol(ISO)-induced myocardial infarction by antagonizing myocardial cell apoptosis.Methods:Thirty male SD rats were randomly divided into three groups:control group(Control),model group(ISO),and Shexiang Baoxin Pill intervention group(ISO+SBP).An acute myocardial infarction rat model was established by intraperitoneal injection of ISO[50 mg/(kg·d)]for 2 days.The ISO+SBP group was administered SBP at a dose of 45 mg/(kg·d)dissolved in distilled water via gavage,while the ISO and Control groups were given an equal volume of distilled water.After 6 weeks of intervention,the rats were sacrificed.Myocardial collagen deposition was detected using Masson staining,apoptosis of myocardial cells was observed using Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling(TUNEL)staining,and the expression levels of Collagen Ⅲ,α-smooth muscle actin(α-SMA),Bax,Bcl-2,Bcl-xl,Caspase-3,and Caspase-9 proteins in myocardial tissue were measured using Western Blotting.Results:Compared with the control group,the ISO group showed significant collagen deposition and myocardial fibrosis,a marked increase in the number of apoptotic cells in the myocardium,and a significant increase in the expression of α-SMA,Collagen Ⅲ,Bax,Caspase-3,and Caspase-9(P＜0.05),with a significant decrease in the expression of Bcl-2 and Bcl-xl(P＜0.05).Compared with the ISO group,the ISO+SBP group exhibited a significant reduction in collagen deposition and apoptosis,as well as a significant decrease in the expression of α-SMA,Collagen Ⅲ,Bax,Caspase-3,and Caspase-9,and a significant increase in the expression of Bcl-2 and Bcl-xl,with statistically significant differences(P＜0.05).Conclusion:Shexiang Baoxin Pill can ameliorate myocardial fibrosis in rats with myocardial infarction,and its mechanism may be associated with the regulation of the apoptosis signaling pathway mediated by Bcl-2 family proteins and Caspases.
Keywords：myocardial infarctionmyocardial fibrosisShexiang Baoxin Pillcell apoptosisexperimental study
Publication Date：2025-02-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 359-363 )