Effect of Phillyrin on Cardiomyocyte Injury Induced by High Glucose Through Regulating cAMP/PKA Signaling Pathway

LIU Yang; ZHU Tao; LI Quanlei; WANG Guangyan

doi:10.12102/j.issn.1672-1349.2025.01.008

Abstract：Objective:To explore the effect of phillyrin on myocardial cell injury induced by high glucose through regulating cyclic adenosine phosphate(cAMP)/cAMP-dependent protein kinase A(PKA)signaling pathway.Methods:H9c2 cardiomyocytes were cultured in vitro and developed into control group,high glucose group,high glucose+low-dose phillyrin group,high glucose+high-dose phillyrin group,and high glucose+high-dose phillyrin+H-89(cAMP inhibitor)group.Cell proliferation was detected by CCK-8.The levels of malondialdehyde(MDA),superoxide dismutase(SOD),and lactate dehydrogenase(LDH)were determined by the kit.The levels of interleukin(IL)-1β,IL-10,and tumor necrosis factor(TNF)-α were determined by enzyme-linked immunosorbent assay.The apoptosis rate was detected by flow cytometry.The expression of B-cell lymphoma/leukemia-2(Bcl-2)and Bcl-2 associated X protein(Bax)were detected by Western Blot.Results:Compared with the control group,the proliferation rate,SOD,IL-10,Bcl-2,cAMP,and P-PKA/PKA protein levels of H9c2 cells in high glucose group decreased,and the levels of MDA,LDH,IL-1β,TNF-α,apoptosis rate,and Bax protein increased(P＜0.05).Compared with high glucose group,the proliferation rate,SOD,IL-10 levels,Bcl-2,cAMP,and p-PKA/PKA protein levels of H9c2 cells in low dose phillyrin group and high glucose+high dose phillyrin group increased,while the levels of MDA,LDH,IL-1β,TNF-α,apoptosis rate,and Bax protein decreased(P＜0.05).H-89 reversed the protective effect of phillyrin on myocardial cell injury induced by high glucose,compared with high glucose+high dose phillyrin group.Conclusion:Phillyrin can reduce the oxidative stress,inflammatory response,and apoptosis rate of high glucose induced cardiac H9c2 cells,promote cell proliferation,and improve cell damage by activating the cAMP/PKA signaling pathway.