Effect of Shexiang Tongxin Dropping Pill on Oxidative Stress of Cardiac Microvascular Endothelial Cells Induced by Advanced Glycation End Products

ZHAO Jian

doi:10.12102/j.issn.1672-1349.2025.01.007

Abstract：Objective:To investigate the effect of Shexiang Tongxin Dropping Pill on oxidative stress in cardiac microvascular endothelial cells(CMECs)induced by advanced glycation end products(AGEs).Methods:CMECs were isolated and cultured from mice,AGEs were used to simulate the damage of high glucose toxicity in CMECs,while Shexiang Tongxin Dropping Pill was used as an intervention drug.Cells were divided into control group,AGEs group,and AGEs+Shexiang Tongxin Dropping Pill group.The concentration of H2S in each group of cells was detected by Methylene Blue method,the level of intracellular reactive oxygen species(ROS)was determined by DCF method.Western Blot was used to detect the relative expression changes of proteins in cells.Real-time fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to detect mRNA relative expression in cells.Enzyme-linked immunosorbent assay(ELISA)was used to determine the oxidative stress related indexes such as glutathione reductase(GSH-Px),superoxide dismutase(SOD)and catalase(CAT)in cells.Results:Shexiang Tongxin Dropping Pill increased H2S production in CMECs and inhibited ROS levels in cells(P＜0.05).Shexiang Tongxin Dropping Pill up-regulated the expression of nuclear factor E2-related factor 2(Nrf2)in CMECs,and the expression of Nrf2 downstream antioxidant genes HO1 and NQO1 were also up-regulated(P＜0.05).The contents of GSH-Px,SOD,CAT and other antioxidant enzymes in cells were increased by Shexiang Tongxin Dropping Pill(P＜0.05).Conclusion:Shexiang Tongxin Dropping Pill can significantly up-regulate the expression of Nrf2 by increasing the production of H2S,thereby increasing the generation of antioxidant enzymes to inhibit cellular oxidative stress and improve microcirculation.