Effect of Luhong Granule on Cardiac and Renal Function in Rats with Type Ⅱ Cardio-renal Syndrome

LIU Qian; WANG Xinting; CHENG Peipei; YANG Tianshu; RONG Jingfeng; ZHOU Hua

doi:10.12102/j.issn.1672-1349.2025.01.006

Abstract：Objective:To investigate the protective effect of Luhong granules on cardiac renal function in rats with type Ⅱ cardio-renal syndrome.Methods:Coronary artery ligation combined with unilateral nephrectomy was used to prepare a type Ⅱ rat model with cardio-renal syndrome.After successful modeling,the rats were randomly divided into sham group,model group,Luhong Granule group and valsartan group.Luhong Granule group was given Luhong Granule(8 g/kg)gavage,valsartan group was given valsartan(8 mg/kg)gavage,the sham operation group and model group were given equal volume of normal saline gavage,and the intervention lasted for a total of 8 weeks.Left ventricular ejection fraction(LVEF)and left ventricular short axis shortening rate(LVFS)were measured by echocardiography.The serum levels of brain natriuretic peptide(BNP)and cystatin C(Cys-C)were detected.Serum creatinine(Scr)was detected by sarcosine oxidase method.The content of blood urea nitrogen(BUN)was detected by urease method.Hematoxylin-eosin(HE)and picric acid-Sirius red staining were used to observe the pathological changes and fibrosis of the heart and kidney in rats.Results:Compared with model group,LVEF and LVFS in Luhong Granule group increased(P＜0.01),while BNP,Scr,BUN,and Cys-C levels decreased(P＜0.01).HE staining and picric acid-Sirius red staining showed that the pathological damage of heart and kidney tissue in Luhong Granule group reduced significantly compared with that in the model group,and the degree of fibrosis reduced.Conclusion:Luhong Granules can increase LVEF and LVFS,decrease the levels of BNP,Scr,BUN and Cys-C,and improve the cardiac and renal function of rats with type Ⅱcardio-renal syndrome,the mechanism may be related to the improvement of fibrosis.