Effect of Breviscapine on β-amyloid across Blood-brain Barrier of Alzheimer's Disease Rats Via Cellular Transporter Pathway
LI Ke
MIAO Guo
LIU Qiu
ZHAO Kunpeng
Abstract：Objective:To investigate the effect of breviscapine onβ-amyloid(Aβ)across blood-brain barrier(BBB)in rats with Alzheimer's disease(AD)rats and its mechanism.Methods:Seventy specific pathogen free(SPF)SD rats were randomly divided into sham operation group,model group,scutellarin low-dose group,scutellarin high-dose group and positive control group.Except for sham operation group,the AD rat model was established by injecting Aβ1-42 sodium chloride solution into the lateral ventricle of the other groups.Scutellarin low-dose group and scutellarin high-dose group were gavaged scutellarin with 5 mg/kg and 10 mg/kg for 30 days respectively,and positive control group was gavaged donepezil hydrochloride with 0.9 mg/kg for 30 days.The behavioral ability and BBB permeability of rats were observed,and hematoxylin-eosin staining(HE)and immunohistochemical staining were performed.Matrix metalloproteinase-9(MMP-9),closure protein-5(Claudin-5),Occludin,low density lipoprotein-related protein(LRP-1)and P-glycoprotein(P-gp)were detected by Western Blot.Results:In the model group,the neuronal cells arranged loosely and disorganized,the number of cells reduced,and the vacuolation was obvious.The number of neuronal cells increased in the scutellarin low-dose group,scutellarin high-dose group and positive control group,and the number of vacuolated cells reduced,and the arrangement of the cells was slightly neat and dense.The escape latency,Evans blue content,Aβ plaque area and relative expression of MMP-9 protein in the scutellarin low-dose group,scutellarin high-dose group and positive control group were lower than those in model group.The frequency of crossing the platform,the relative expression of LRP-1,P-gp,Claudin-5 and Occludin were higher than those in the model group(P<0.05).Conclusion:Breviscapine could alleviate pathological damage of neurons and protect BBB in rats with AD.
Keywords：Alzheimer's diseaseblood-brain barrierβ-amyloidbreviscapineexperimental study
Publication Date：2024-05-16
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1782-1786 )