Protective Effect of Shexiang Tongxin Dropping Pill Pretreatment on Myocardial Ischemia-reperfusion Injury in Rats
LI Meng
LIU Yong
ZHANG Jianzhen
HAO Xuezeng
ZHANG Lijing
Abstract：Objective:To observe the protective effect of Shexiang Tongxin Dropping Pills on myocardial cells and blood vessels after myocardial ischemia-reperfusion(I/R)injury in rats.Methods:Sixty male SD rats were randomly divided into sham operation group(Sham group),I/R group,Shexiang Tongxin Dropping Pills group(SXTX group),and positive control group,with 15 rats in each group.The rats in SXTX group were given SXTX 18.90 mg/(kg·d)by gavage,and the rats in positive control group were given nicorandil 1.35 mg/(kg·d)by gavage.The rat in Sham group and I/R group were given the same volume of deionized water by gavage.After one week of gavage,the rats left anterior descending coronary artery was ligated for 30 min and then reperfused for 120 min to build the myocardial I/R model except for the Sham group.The changes of myocardial tissue and blood vessels in rats were observed by myocardial hematoxylin-eosin(HE)staining and myocardial Masson staining.The ultrastructural changes of cardiomyocytes and blood vessels were observed by electron microscope.The expressions of hypoxia-inducible factor-1α(HIF-1α)and vascular endothelial growth factor(VEGF)were observed by immunohistochemistry.Results:Compared with Sham group,the myocardial cell necrosis and vascular injury of the rats in I/R group were obvious,and the expression of HIF-1αin the myocardial tissue increased.Compared with I/R group,the myocardial cell and vascular pathological structural damage in SXTX group alleviated,and the protein expressions of VEGF in the ischemic area significantly increased.Conclusion:Shexiang Tongxin Dropping Pills pretreatment can protect myocardial cells and vascular I/R injury in rats,which may be related to the up-regulation expression of VEGF protein and the promotion of microangiogenesis in ischemic areas.
Keywords：ischemia/reperfusion injuryShexiang Tongxin Dropping Pillsprotective effecthypoxia-inducible factor-1αvascular endothelial growth factorexperimental study
Publication Date：2024-01-16
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 250-253 )