Metabonomics Study of Huoxue Yixin Formula for the Treatment of Qi Deficiency and Blood Stasis Syndrome of Acute Coronary Syndrome
FENG Juan
ZHU Cuiling
DUAN Fei
SUN Yanqin
XIE Mengxin
LIU Xiaomeng
LIU Gang
YAN Kuipo
Abstract：Objective:To explore the mechanism of Huoxue Yixin Formula for the treatment of Qi deficiency and blood stasis syndrome of acute coronary syndrome.Methods:According to the random digital table,13 patients with acute coronary syndrome were randomly divided into treatment group and control group.The serum metabolites of two groups before and after treatment were detected by high performance liquid chromatography-mass spectrometry.The metabolic profiles of two groups were compared with patients without coronary heart disease to screen out the potential metabolites,and metabolic pathways associated with disease treatment were then predicted.Results:Patients with acute coronary syndrome had some specific metabolic spectrum,which was significantly different from patients without coronary heart disease.Compared with the control group,the expression levels of 9 substances such as ursodeoxycholic acid,sphinganine,methylmalonic acid significantly up-regulated,and the expression levels of 21 substances such as norepinephrine,pyruvic acid,L-tryptophan significantly down-regulated after the intervention of Huoxue Yixin Formula.Differential metabolite pathway analysis showed that the mechanism of Huoxue Yixin Formula for the treatment of acute coronary syndrome mainly related to typeⅡ diabetes mellitus,hypoxia inducible factor 1(HIF-1)signaling pathway,cyclic adenosine monophosphate(cAMP)signaling pathway,nicotinate and nicotinamide metabolism,cholesterol metabolism,pantothenate and coenzyme A(CoA)biosynthesis,citrate cycle,adrenergic signaling in cardiomyocytes,oxidative phosphorylation.Conclusion:Thirty potential biomarkers were identified,involving 15 metabolic pathways.This study revealed what happened in the body after the intervention of the disease and traditional Chinese medicine by metabolomics technology,and elaborated the mechanism of Huoxue Yixin Formula for the treatment of acute coronary syndrome from the level of metabolic end products.It provided a certain reference for the follow-up trials,the research and development of new drugs.
Keywords：acute coronary syndromeHuoxue Yixin Formulametabonomicshigh performance liquid chromatography-mass spectrometry
Publication Date：2024-01-16
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：12( 201-212 )