Mechanism of Galangin on Isoprenaline-induced Cardiac Fibrosis
SHANG Ruru
TONG Manlin
LIU Xiaohong
Abstract：Objective:To investigate the mechanism of Galangin on isoprenaline(ISO)-induced cardiac fibrosis.Methods:Thirty C57BL/6 mice were randomly divided into the normal group,ISO group,low-dose galangin group[25 mg/(kg·d)],medium-dose galangin group[50 mg/(kg·d)],and high-dose galangin group[100 mg/(kg·d)].After treatment,cardiac ultrasound was performed to detect cardiac function[left ventricular ejection fraction(LVEF),left ventricular short-axis shortening(LVFS),left ventricular end-systolic internal diameter(LVESD),and left ventricular end-diastolic internal diameter(LVEDD)].The body mass(BW),heart weight(HW),and tibia length(TL)of the mice were measured,and the heart weight-weight to body mass ratio(HW/BW)was detected,and the heart weight to tibia length ratio(HW/TL)were collected to assess cardiac hypertrophy.The degree of cardiac fibrosis was detected by sirius scarlet staining.The inflammatory markers interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),and fibrotic markers collagenⅠ(CollagenⅠ),collagenⅢ(CollagenⅢ),transforming growth factor-β1(TGF-β1)mRNA levels were detected by real-time fluorescence quantitative polymerase chain reaction(qPCR).The kit was used to detect the oxidative stress indicators superoxide dismutase(SOD),catalase(CAT)activity,and malondialdehyde(MDA)content in heart tissue.Results:Compared with the normal group,HW/BW,HW/TL,LVEDD,and LVESD in the ISO group were higher,LVEF and LVFS were less,levels of IL-6,IL-1β,TNF-α,Collagen Ⅰ,Collagen Ⅲ,and TGF-β1 mRNA were higher,SOD and CAT activities were less,and MDA content was higher.The cardiomyocyte cross-sectional area and cardiac fibrosis area were higher(P<0.05).Compared with the ISO group,the area of cardiac fibrosis was less in the high-dose galangin group,the expression levels of Collagen Ⅰ,Collagen Ⅲ,and the mRNA of IL-6 were less,the activity of SOD was higher,and the content of MDA was less(P<0.05).TGF-β1 in the medium-dose galangin group was less than that in the ISO group(P<0.05).Conclusion:Galangin could alleviate isoproterenol-induced cardiac fibrosis,and its mechanism might be related to inhibiting inflammation and oxidative stress.
Keywords：cardiac fibrosisGalanginisoproterenolinflammationoxidative stressmiceexperimental study
Publication Date：2023-12-16
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 4524-4529 )