Establishment of Hypertensive Rat Models with Phlegm-damp Accumulation

ZHU Fuzhen; YIN Yanyan; LIANG Jinjie; GUO Songran; XU Zhonghui; WANG Lingzhi

doi:10.12102/j.issn.1672-1349.2023.24.008

Abstract：Objective:To establish rapid and stable hypertensive rat models with phlegm-damp accumulation.Methods:Three hypertensive rat models with phlegm-damp accumulation were established by different high-fat diet-induced spontaneously hypertensive rats(SHR).SHR were randomly divided into 4 groups:SHR control group,model Ⅰ group,model Ⅱ group and model Ⅲgroup,and Wistar rats were used as normal control group.Two control groups were fed with normal diet for 8 weeks,and three model groups were fed with high-fat diet 1,high-fat diet 2,and high-fat diet 3,respectively for 8 weeks.Behavioral changes of SHR were monitored during the modeling process,serum lipid were analyzed by fully automated biochemistry,and blood pressure changes were detected by the tail-cuff method.Ultrasound method was used to detected the SHR's cardiac structure.Results:The blood pressure of the model Ⅰ group,which was established by feeding with the high-fat diet 1(egg yolk powder 10%,sucrose 10%,lard 15%,cholesterol 1.2%,sodium cholate 0.2%,appropriate amounts of casein,calcium hydrogen phosphate,and stone powder),systolic blood pressure was stably maintained above 167.8 mmHg.The body weight and low-density lipoprotein cholesterol(LDL-C)increased(P<0.05),and high-density lipoprotein cholesterol(HDL-C)decreased(P<0.05),and the success rate of modeling was 100%.Cardiac ultrasound showed that compared with Wistar rats,the left ventricular anterior wall at end-systole(LVAWs),left ventricular posterior wall at end-diastole(LVPWd),and left ventricular mass average weight(LV Mass AW)increased(P<0.05),and the left ventricular internal diameter at end-diastole(LVIDd)of the SHR control group and the modelⅠ group decreased(P<0.05),while the liver index of the modelⅠgroup was significantly higher than that of the SHR control group(P<0.05).Conclusion:By a high-fat diet with multiple fat sources HSR could be used to establish rapid and stable hypertensive rat models with obstructed phlegm-damp accumulation that fulfilled clinical requirements.