Effect of Codonopsis Pilosula Polysaccharide on Neurological Function and Cerebral Protection in Mice with Type Ⅰ Herpes Simplex Virus Encephalitis Based on PI3K/AKT/GSK3β Pathway
HUANG Hong
AN Juyan
FAN Xinli
Abstract：Objective:To observe the effect of codonopsis pilosula polysaccharide on the neurological function and cerebral protection of type I herpes simplex encephalitis(HSE)mice,and to explore the possible mechanism.Methods:All 50 BALB/c mice and 40 mice were selected to establish the type Ⅰ HSE model.Excluding the mice that died during the experiment,the remaining 30 mice were randomly divided into HSV-Ⅰ group,codonopsis pilosula polysaccharide group and combined group,with 10 mice in each group.The other 10 mice were set as the healthy group.At the day after modeling,mice in the combined group were gavaged with codonopsis pilosula polysaccharide(100 mg/kg)and intraperitoneally injected with LY294002[phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/glycogen synthase kinase 3β(GSK-3β)inhibitor]30 mg/kg.The mice in the codonopsis pilosula polysaccharide group were intragastrically administered with codonopsis pilosula polysaccharide(100 mg/kg),and an equal volume of dimethyl sulfoxide(DMSO)was injected intraperitoneally.The mice in the healthy group and HSV-Ⅰ group were intragastrically injected with the same volume of normal saline and intraperitoneally injected with the same volume of DMSO.Once a day for 7 days.Serum levels of inflammatory factors,oxidative stress factors and neurological function indexes were tested.Virus titers in brain,liver and lung tissues were tested.Western Blot was used to detect the expression of AKT,p-AKT,GSK3β,and p-GSK3βproteins in cerebral tissue.Results:Compared with the HSV-Ⅰ group,the serum interleukin-1β(IL-1β),nitric oxide(NO),malondialdehyde(MDA),reactive oxygen species(ROS),interferonγ(IFN-γ),and calcium binding protein(S-100B)level decreased,superoxide dismutase(SOD)activity level,cerebral tissue p-AKT/AKT,p-GSK3β/GSK3βincreased,brain,liver,and lung tissue virus titers decreased in the codonopsis pilosula polysaccharide group(P＜0.05).Compared with the codonopsis pilosula polysaccharide group,serum IL-1β,NO,MDA,ROS,IFN-γ,and S-100B levels increased,SOD activity level,brain tissue p-AKT/AKT,p-GSK3β/GSK3β decreased,brain,liver and lung tissue virus titers increased in the combined group(P＜0.05).Conclusion:Codonopsis pilosula polysaccharide can inhibit inflammation and oxidative stress,reduce nerve and cerebral tissue damage,and inhibit HSV-Ⅰ virus replication.It is speculated that its mechanism of action is related to the activation of PI3K/AKT/GSK3βsignaling pathway.
Keywords：type Ⅰ herpes simplex virus encephalitiscodonopsis pilosula polysaccharideprotein kinase Bglycogen synthase kinase 3β
Publication Date：2023-12-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 4347-4352 )