Effect of Liraglutide Up Regulation of miR-93-5p on the Injury of Hypoxic/Reoxygenated H9c2 Cardiomyocytes
CAO Guanglin
LIU Bin
LIU Na
Abstract：Objective:To investigate the protective effect of liraglutide up-regulation of miR-93-5p on hypoxic/reoxygenated H9c2 cardiomyocyte injury.Methods:Logarithmic H9c2 cardiomyocytes were divided into control group,hypoxia/reoxygenation group,liraglutide group,anti-miR-93-5p group,and anti-miR-93-5p+liraglutide group.Cells in anti-miR-93-5p group and anti-miR-93-5p+ liraglutide group were transfected with anti-miR-93-5p 50μmol/L,24 h later,anti-miR-93-5p+liraglutide group,liraglutide group were added to liraglutide 100μmol/L and pretreated for 12 h.Except the control group,anoxia/reoxygenation model was established in the other groups.Myocardial cell survival rate,myocardial cell apoptosis rate,oxidative stress index[malondialdehyde(MDA)content,superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)]activity,miR-93-5p expression,activated cysteine aspartase 3(Cleaved),expression levels of Caspase-3,and blymphoblastoma-2 gene(Bcl-2)and Bcl-2 associated X protein(Bax)were detected and compared between all groups.Results:Compared with the control group,myocardial cell survival rate,SOD,GSH-Px activity,miR-93-5p level,and Bcl-2 protein expression decreased in hypoxia/reoxygenation group,and apoptosis rate,MDA content,Cleaved Caspase-3,and Bax protein expression increased(P＜0.05).Compared with hypoxia/reoxygenation group,myocardial cell survival rate,SOD,GSH-Px activity,miR-93-5p level,and Bcl-2 protein expression increased in liraglutide group,while myocardial cell apoptosis rate,MDA content,Cleaved Caspase-3,and Bax protein expression decreased;in anti-miR-93-5p group,the survival rate of cardiomyocytes,SOD,GSH-Px activity,miR-93-5p level,and Bcl-2 protein expression decreased,and the apoptosis rate,MDA content,Cleaved Caspase-3,and Bax protein expression increased(P＜0.05).Compared with liraglutide group,the survival rate of cardiomyocytes,SOD,GSH-Px activities,miR-93-5p level,and Bcl-2 protein expression in anti-miR-93-5p+liraglutide group decreased,apoptosis rate,MDA content,Cleaved Caspase-3,and Bax protein expression increased(P＜0.05).Compared with anti-miR-93-5p group,the survival rate of cardiomyocytes,SOD,GSH-Px activities,miR-93-5p level,and Bcl-2 protein expressionin anti-miR-93-5p+liraglutide group increased,apoptosis rate,MDA content,Cleaved Caspase-3 and Bax protein expression decreased(P＜0.05).Conclusion:Liraglutide can ameliorate the injury of hypoxic/reoxygenated cardiomyocytes,possibly through up-regulating the expression of miR-93-5p,reducing the level of cellular oxidative stress,and reducing cell apoptosis.
Keywords：hypoxia/reoxygenationliraglutidemiR-93-5pcell injuryexperimental study
Publication Date：2023-12-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 4316-4321 )