Ginkgo Biloba and Lepidii Semen Alleviate Myocardial Fibrosis Based on Network Pharmacology Combined with Gene Expression Omnibus Chip Analysis
HUANG Hao
YU Guangbin
CHEN Li
GU Jianhao
HONG Yongdun
Abstract：Objective:To explore the mechanism of the Chinese herb ginkgo biloba and lepidii semen alleviate myocardial fibrosis based on network pharmacology analysis,molecular docking and gene expression omnibus(GEO)chip analysis and verification.Methods:The pharmacologic database and analysis platform of traditional Chinese medicine system were used to screen the effective ingredients and targets of ginkgo biloba and lepidii semen.The target of myocardial fibrosis was obtained by GeneCards and online Mendelian inheritance in man(OMIM).The effective ingredients and myocardial fibrosis targets were introduced into Cytoscape 3.8.0 software to construct drug-ingredient-target network.The STRING platform builded protein-protein interaction(PPI)networks.The network analysis tools-Cytoscape was used to calculate network topology parameters,analyze and obtain key targets.The intersection targets were analyzed by R software for gene ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment.The chip data of GEO was used for data analysis,and the molecular docking of effective ingredients and core targets was carried out by AutoDock software,and the molecular docking model diagram was drawn by Pymol software to verify the core targets.Results:Thirty nine effective ingredients were obtained,116 active ingredient action targets and 3 322 myocardial fibrosis targets were screened.Key targets such as transcription factor P65(RELA),androgen receptor(AR),prostaglandin G/H synthase 1(PTGS1),tumor protein P53(TP53),and nuclear factor-κB(NF-κB)inhibitorα(NFKBIA)were identified by network topology analysis.GO enrichment analysis mainly involved cellular response to chemical stress,response to steroid hormones,DNA binding transcription factor binding,nuclear receptor activity,ubiquitin protein ligase,and other processes.KEGG enrichment analysis mainly included lipid and atherosclerosis,phosphatidylinositol 3-kinase(PI3K)/total protein kinase B(AKT)signaling pathway and mitogen-activated protein kinase(MAPK)signaling pathway,and other pathways.GEO gene chip analysis and verification showed that AR,RELA,TP53,NFKBIA,and PTGS1 were significantly different among the first 6 core targets(P＜0.05).The results of molecular docking showed that the main effective ingredients and the target could spontaneously bind and most of the binding ability was better.Conclusion:Ginkgo biloba and lepidii semen shows anti-inflammatory and anti-oxidative stress effects through NF-κB,MAPK signaling pathway and PI3K/AKT signaling pathway,and other signaling pathways,by inhibiting fibroblast related proliferation and activation process,reducing extracellular matrix deposition,thereby alleviating myocardial fibrosis.
Keywords：myocardial fibrosisginkgo bilobalepidii semenmitogen-activated protein kinase signaling pathwaynuclear factor-κBnetwork pharmacologygene expression
Publication Date：2023-12-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 4279-4288 )