Effect of Bushen Zhichan Decotion and Needle-embedding Therapy on Praxiology and Freeradical Scavenging in Substantia nigra in Rats with Parkinson′s Disease

HU Yuying; LUO Yuxiong; QIAN Kai; MO Haizhen

doi:10.12102/j.issn.1672-1349.2019.02.010

Abstract：Objective To observe the effect of Bushen Zhichan decotion(BZD)and needle-embedding therapy on praxiology and freeradical scavenging in substantia nigra of midbrain in rats with Parkinson′s disease(PD).Methods Forty Sprague-Dawley(SD)rats were randomly divided into the normal control group(n =10)and the model group(n =30).Rats in the model group chronic PD model were established by subcutaneous injection of protease inhibitor.Then,they were randomly divided into the model group,Levodopa and Benserazide group,BZD and needle-embedding group,10rats in each group.The rats were administered with normal saline 1 mL/(100 g ·d)in the model group and sham operation group,Levodopa and Benserazide 200 mg/(kg·d)in the Levodopa and Benserazide group, BZD1 mL/(100 g·d)combined with needle-embedding therapy in BZD and needle-embedding group.Rats in each group were treated on alternate days(every Monday,Wednesday,Friday).After 3 weeks of intervention,the pathological changes of the praxiology,the contents of GSH and LPO,as well as the activity of GSH-Px and SOD in substantia nigra of midbrain in rats were observed.Results Compared to the sham operation group,the contents of GSH and the activity of GSH-Px and SOD in the model group declined(P <0. 01),while the contents of LPO in the model group significantly increased(P <0.01).The mobile latency as well as the over bar time of the model group in the grid test and the balance bar test were prolonged(P <0.01).Compared to the model group,the contents of GSH and the activity of GSH-Px and SOD in BZD and needle-embedding group and Levodopa and Benserazide group significantly increased(P <0.01),which were higher in the combined needle-embedding group than that in the Levodopaand Benserazide group(P<0.01).The contents of LPO declined in BZD and needle-embedding group and Levodopa and Benserazide group(P <0.01),which were lower in the combined needle-embedding group than that in the Levodopaand Benserazide group(P <0.05).The mobile latency and the over bar time were shortened in BZD and needle-embedding group and Levodopa and Benserazide group(P <0.01),which were shorter in the combined needle-embedding group than that in the Levodopa and Benserazide group(P <0.05).Conclusion BZD and needle- embedding therapy can significantly improve the praxiology in rats,and obviously enhance the activity of scavenging free radical in substantia nigra of midbrain,thus protect the dopaminergic neurons in the substantia nigra.