Inhibitory Effect of Salvianolate on Myocardial Apoptosis after Myocardial Ischemia-reperfusion Injury in Rats and Its Mechanism
LI Bing
Abstract：Objective To investigate the inhibitory effect and mechanism of Salvianolate on myocardial apoptosis after myocardial ischemia- reperfusion injury(MIRI)in rats.Methods The myocardial ischemia-reperfusion model was established by clamping the left anterior descending coronary artery.The rats were divided into sham group,model group,and Salvianolate groups(10,20,40 mg/kg,n= 20in each group).The changes of electrocardiogram (ECG)in each group were monitored.The infarct size was detected by 2,3,5-triphenyltetrazolium chloride(TTC)staining method.The myocardial histopathological changes was observed by Hematoxylin-Eosin (HE)staining.The apoptosis of cardiomyocytes was observed by TUNEL.The expression of apoptosis-related proteins was detected by western blotting.Results Compared with model group,The ECG in Salvianolate(10,20,40 mg/kg)groups were significantly improved,especilly in the Salvianolate 40 mg/kg group.The myocardial infarct size in Salvianolate 20 mg/kg group and Salvianolate 40 mg/kg group were significantly decreased(P <0.05 or P <0.01).The myocardial histopathological changes and myocardial cardiomyocyte apoptosis were significantly improved and the apoptotic index(AI)were significantly decreased(P <0.01).The expression of Bcl-2 protein was significantly increased,and the expressions of Bax,and cleaved Caspase-3 were significantly decreased(P <0.05 or P <0.01).The ratio of bcl-2/Bax was significantly increased(P <0.01).Compared with the model group,the expression of anti-apoptotic protein Bcl-2 was significantly up-regulated in the Salvianolate 20 mg/kg group and 40 mg/kg group,and the pro-apoptotic protein Bax expression was significantly down-regulated(P <0.05 or P <0.01).The Bcl-2/Bax ratio was increased(P <0. 01),and the expression of proapoptotic protein-cleaved Caspase-3 protein was significantly down-regulated(P <0.05 or P <0.01). Conclusion Salvianolate has an inhibitory effect on cardiomyocyte apoptosis after MIRI,and regulation of apoptosis-related proteins such as Bcl-2,Bax,and activated Caspase-3 may be an important molecular mechanism.
Keywords：myocardial ischemiareperfusionSalvianolatemyocardial apoptosisinhibitory effectBcl-2Baxcleaved Caspase-3
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 192-195 )