Effect of Momordica Charantia Saponins on Myocardial Apoptosis in Rats with Heart Failure
HUANG Wenwei
HONG Lifeng
GUI Yuran
SU Binbin
GUO Fan
Abstract：Objective To investigate the effects of Momordica charantia saponins(MCS)on the cardiac function and the expression of Caspase-8 and Bcl-2in rats with heart failure.Methods Sixty male Sprague-Dawley(SD)rats were randomly divided into the normal group,model group,MCS low dose group,MCS medium dose group,and MCS high dose group,12 rats in each group.The chronic heart failure model was established by the treatment of isoproterenol hydrochloride.After successful modeling,the rats were administered with subcutaneous injection of MCS 10 mg/(kg·d),20 mg/(kg·d),and 40 mg/(kg·d)in MCS low dose group,MCS medium dose group,and MCS high dose group,respectively.the rats in the normal group and the model group were given with subcutaneous injection of 0.5 mL/(kg·d)saline.The rats were sacrificed after 8 weeks of treatment.The cardiac function related indicators,and inflammatory factors tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)levels were detected.The myocardial histopathological changes were detected by Hematoxylin-Eosin(HE)staining.The expressions of Caspase-8 and Bcl-2 related proteins in myocardium were detected by immunohistochemistry,real-time fluorescence quantitative polymerase chain reaction(qRT-PCR),western blot, and real-time fluorescent PCR.Results The rats in the normal group had a good mental and normal diet,while the rats in model group had obvious apathy,compared with the model group,the above symptoms were significantly reduced.Compared with the normal group,the left ventricular pressure peak(LVSP),+dp/dtmax and-dp/dtmax were significantly decreased in the model group(P <0. 05).Compared with the model group,left ventricular diastolic pressure(LVDP)was significantly decreased(P <0.05),LVSP,+dp/ dtmax and-dp/dtmax were significantly increased in MCS low dose group,MCS medium dose group and MCS high dose group(P <0.05).Compared with the normal group,TNF-αand IL-6in the model group were significantly increased after treatment(P <0.05). Compared with the model group,TNF-α,and IL-6 were significantly decreased in MCS low dose group,MCS medium dose group and MCS high dose group after treatment(P <0.05).HE staining showed that the cardiomyocytes were neatly arranged in the normal group,and had obvious disorder in the model group.The myocardial tissue in MCS low dose group,MCS medium dose group and MCS high dose group had different degrees of improvement compared with the model group.Compared with the normal group,the expression of Bcl-2 protein in the model group was significantly lower than that in the normal group(P <0.05),and the expression of Caspase-8 protein in the model group was significantly higher than that in the normal group(P <0.05).Compared with the model group,the expression of Bcl-2 protein in MCS groups were increased(P <0.05),Caspase-8 expression was significantly decreased(P <0.05). Conclusion MCS can significantly improve the cardiac function and decrease the expression of inflammatory factors TNF-αand IL-6 in rats with chronic heart failure,which may be related to down-regulating Caspase-8 and up-regulating Bcl-2 expression.
Keywords：chronic heart failureMomordica charantia saponincardiac functioncardiomyocyte apoptosisCaspase-8Bcl-2tumor necrosis factor-αinterleukin-6
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 186-191 )