Research on the Influence Factors of H9c2 Cardiomyocytes Glycolysis and Mitochondrial Respiratory Function in Rats
LI Bin
CUI Lin
WANG Xinlu
WANG Youping
XIE Shiyang
HAO Xuanxuan
GAO Yuan
WANG Xiaoxiao
ZHU Mingjun
Abstract：Objective This study was designed to explore the influence factors on H9 c2 cells energy metabolism with cellular energy metab-olism detection system (Seahorse XF Analyzer).Methods Different cell density of H9 c2 rat myocardial cell were cultured in SeahorseXF24 orifice plate, glycolysis was detected by glycolysis kits.With 105/mL cell density, different concentration FCCP stimulate cells, mito-chondrial function was detected using mitochondrial aerobic oxidation kits.Results The density of cells respond well to 2×104 cell numberof oligomycin stimulation rate value was better.50-400 range was the more appropriate cell density.2μmol/L FCCP could stimulate themaximum reaction, which was more appropriate concentration.Conclusion The cell density and FCCP concentration are the main factorsinfluencing the determination of cardiomyocytes glycolysis and mitochondrial respiratory.H9 c2 cardiomyocytes in 2×104/well cell inoculationdensity, and FCCP concentration 2μmol/L stimulate cells will be suitable for mitochondrial function test.
Keywords：Shenfu Yixin particlescardiomyocytesenergy metabolismmitochondrial respiratoryglycolysis
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 60-64 )